p-GSK3α (S21) pAb

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h3 class=list6_t1Product/h3
p class=list6_text1 mg/ml in Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.2./p
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h3 class=list6_t1Purification amp; Purity/h3
p class=list6_textThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is gt; 95% (by SDS-PAGE)./p
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h3 class=list6_t1Specificity/h3
p class=list6_textp-GSK3alpha; (S21) antibody detects endogenous levels of p-GSK3alpha; protein./p
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h3 class=list6_t1Applications (Recommended Dilutions)/h3
p class=list6_textWB: 1:500~1:1000 IHC: 1:50~1:200 IP:1:50~1:200/p
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h3Western Blot(WB)/h3
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div style=width: 250pxWestern blot (WB) analysis of p-GSK3alpha; (S21) antibody antibody in extracts from ovary cancer./div
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h3Immunohistochemistry (IHC)/h3
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pImmunohistochemistry (IHC) analyzes of p-GSK3alpha; (S21) Antibody in paraffin-embedded human breast carcinoma tissue/p
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h3 class=list6_t1Storageamp;Stability/h3
p class=list6_textStore at 4deg;C short term. Aliquot and store at -20deg;C long term. Avoid freeze-thaw cycles./p
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h3 class=list6_t1Background/h3
p class=list6_textGlycogen synthase kinase-3 alpha; and beta; (GSK-3alpha;, beta;) are serine/threonine kinases that regulate metabolic enzymes and transcription factors, which are responsible for coordinating processes such as glycogen synthesis and cell adhesion. GSK-3beta; activity is also required for nuclear activity of Rel dimers, which mediate an anti-apoptotic response to TNFalpha; in mice. GSK-3 catalytic kinase activity is controlled through differential phosphorylation of serine/threonine residues, which have an inhibitory effect, and tyrosine residues, which have an activating effect. Growth factor stimulation of mammalian cells expressing GSK-3alpha; and GSK-3beta; induces phosphorylation of Ser 21 and Ser 9, respectively through a phosphatidylinositol 3-kinase (PI 3-kinase)-protein kinase B (PKB) dependent pathway, thereby enhancing proliferative signals. Additionally, GSK-3 physically associates with cAMP-dependent protein kinase A (PKA), which phosphorylates Ser 21 of GSK-3alpha; or Ser 9 of GSK-3beta; and inactivates both forms/p
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