p-FAK (Y861) pAb
- 貨號 Cat. No.
- BS4719
- 規格 Size
- 100 μg
- 品牌 Brand
- Bioworld
Description
div class=list6_content
div class=list6_li
h3 class=list6_t1Product/h3
p class=list6_text1 mg/ml in Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.2./p
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div class=list6_li
h3 class=list6_t1Purification amp; Purity/h3
p class=list6_textThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is gt; 95% (by SDS-PAGE)./p
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h3 class=list6_t1Specificity/h3
p class=list6_textp-FAK (Y861) antibody detects endogenous levels of p-FAK protein./p
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h3 class=list6_t1Applications (Recommended Dilutions)/h3
p class=list6_textWB: 1:500~1:1000/p
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div id=westernpic
div class=imagecontainer
h3Western Blot(WB)/h3
img id=wpic src=http://www.bioworlde.com/upload/web/4719.jpg width=120 alt= /
div style=width: 250pxWestern blot (WB) analyzes of p-FAK (Y861)antibody in extracts from Jurkat cells./div
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h3 class=list6_t1Storageamp;Stability/h3
p class=list6_textStore at 4/p
/div
div class=list6_li
h3 class=list6_t1Background/h3
p class=list6_textActivation of integrins in the extracellular matrix (ECM) of eukaryotic cells promotes the formation of membrane adhesion complexes, known as focal adhesions, which can include cytoskeletal proteins and protein tyrosine kinases, such as focal adhesion kinase (FAK). Phosphorylation events occurring within focal adhesions influence numerous processes that include mitogenic signaling, cell survival and cell motility. FAK is a non-receptor tyrosine kinase that is ubiquitously expressed and highly conserved between species. FAK is recruited by integrin clusters and variably phosphorylated depending on the effector molecules present in the focal adhesion. Phosphorylation of Ser 722 is unchanged in unsynchronized and mitotic cells whereas Ser 843 and Ser 910 phosphorylation is elevated during mitosis. Ser 722 phosphorylation within the concensus type II polyproline motif of FAK, may negatively regulate the ability of FAK to associate with an SH3 domain in p130 Cas./p
/div
/div
div class=list6_li
h3 class=list6_t1Product/h3
p class=list6_text1 mg/ml in Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.2./p
/div
div class=list6_li
h3 class=list6_t1Purification amp; Purity/h3
p class=list6_textThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is gt; 95% (by SDS-PAGE)./p
/div
div class=list6_li
h3 class=list6_t1Specificity/h3
p class=list6_textp-FAK (Y861) antibody detects endogenous levels of p-FAK protein./p
/div
div class=list6_li
h3 class=list6_t1Applications (Recommended Dilutions)/h3
p class=list6_textWB: 1:500~1:1000/p
/div
div id=pic
div id=westernpic
div class=imagecontainer
h3Western Blot(WB)/h3
img id=wpic src=http://www.bioworlde.com/upload/web/4719.jpg width=120 alt= /
div style=width: 250pxWestern blot (WB) analyzes of p-FAK (Y861)antibody in extracts from Jurkat cells./div
/div
/div
/div
div class=list6_li
h3 class=list6_t1Storageamp;Stability/h3
p class=list6_textStore at 4/p
/div
div class=list6_li
h3 class=list6_t1Background/h3
p class=list6_textActivation of integrins in the extracellular matrix (ECM) of eukaryotic cells promotes the formation of membrane adhesion complexes, known as focal adhesions, which can include cytoskeletal proteins and protein tyrosine kinases, such as focal adhesion kinase (FAK). Phosphorylation events occurring within focal adhesions influence numerous processes that include mitogenic signaling, cell survival and cell motility. FAK is a non-receptor tyrosine kinase that is ubiquitously expressed and highly conserved between species. FAK is recruited by integrin clusters and variably phosphorylated depending on the effector molecules present in the focal adhesion. Phosphorylation of Ser 722 is unchanged in unsynchronized and mitotic cells whereas Ser 843 and Ser 910 phosphorylation is elevated during mitosis. Ser 722 phosphorylation within the concensus type II polyproline motif of FAK, may negatively regulate the ability of FAK to associate with an SH3 domain in p130 Cas./p
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