p-ER-a (S104) pAb

div class=list6_content
div class=list6_li
h3 class=list6_t1Product/h3
p class=list6_text1 mg/ml in Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.2./p
/div
div class=list6_li
h3 class=list6_t1Purification amp; Purity/h3
p class=list6_textThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is gt; 95% (by SDS-PAGE)./p
/div
div class=list6_li
h3 class=list6_t1Specificity/h3
p class=list6_textp-ER-a (S104) antibody detects endogenous levels of p-ER-a protein./p
/div
div class=list6_li
h3 class=list6_t1Applications (Recommended Dilutions)/h3
p class=list6_textIHC: 1:50~1:200/p
/div
div id=pic
div id=IHCpic
div class=imagecontainer
h3Immunohistochemistry (IHC)/h3
img id=ipic src=http://www.bioworlde.com/upload/ihc/5067.jpg width=240 height=180 alt= //div
pImmunohistochemistry (IHC) analyzes of p-ER-a (S104) antibody in paraffin-embedded human breast carcinoma tissue./p
/div
/div
div class=list6_li
h3 class=list6_t1Storageamp;Stability/h3
p class=list6_textStore at 4deg;C short term. Aliquot and store at -20deg;C long term. Avoid freeze-thaw cycles./p
/div
div class=list6_li
h3 class=list6_t1Background/h3
p class=list6_textThe estrogen receptor-alpha (ER) is a broadly expressed transcription factor which controls a number of genes involved in cellular differentiation and proliferation. ER contains two transcriptionally active domains: the N-terminal domain (AF1) is constitutively active, while the C-terminal domain (AF-2) is hormone-dependent. ER is subject to post-translational modification, including phosphorylation of serine residues 104, 106, 118, and 167. This antibody specifically recognizes human estrogen receptor-alpha when phosphorylated at serine 167, a site which influences AF-1 dependent transcriptional activity. This phosphorylation is catalyzed by the MAPK pathway, potentially through the 90 kDa ribosomal S6 kinase (pp90rsk1). Serine 167 is also a consensus Akt substrate./p
/div
/div