p-eIF4E (S209) pAb

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h3 class=list6_t1Product/h3
p class=list6_text1 mg/ml in Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.2./p
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h3 class=list6_t1Purification amp; Purity/h3
p class=list6_textThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is gt; 95% (by SDS-PAGE)./p
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h3 class=list6_t1Specificity/h3
p class=list6_textp-eIF4E (S209) antibody detects endogenous levels of p-eIF4E protein./p
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h3 class=list6_t1Applications (Recommended Dilutions)/h3
p class=list6_textWB: 1:500~1:1000 IHC: 1:50~1:200/p
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h3Western Blot(WB)/h3
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div style=width: 250pxWestern blot (WB) analysis of p-eIF4E (S209) antibody in extracts from NIH/3T3 FBS cells ./div
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h3Immunohistochemistry (IHC)/h3
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pImmunohistochemistry (IHC) analyzes of p-eIF4E (S209) antibody in paraffin-embedded human breast carcinoma tissue./p
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h3 class=list6_t1Storageamp;Stability/h3
p class=list6_textStore at 4deg;C short term. Aliquot and store at -20deg;C long term. Avoid freeze-thaw cycles./p
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h3 class=list6_t1Background/h3
p class=list6_textThe initiation of protein synthesis in eukaryotic cells is regulated by interactions between protein initiation factors and RNA molecules. The eukaryotic initiation complex eIF4F exists in vitro as a trimeric complex of eIF4G, eIF4E, and eIF4A. Together, the complex allows ribosome binding to mRNA by inducing the unwinding of mRNA secondary structures. eIF4E is a 25 kDa protein that binds to the mRNA ldquo;caprdquo; during an early step in the initiation of protein synthesis. eIF4A is a 46 kDa protein that acts as an ATP-dependent RNA helicase. eIF4G is 154 kDa protein that acts as a bridge between eIF4E, eIF4A, and the eIF3 complex./p
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