p-Cdc25A (S75) pAb
- 貨號 Cat. No.
- BS4715
- 規格 Size
- 100 μg
- 品牌 Brand
- Bioworld
Description
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h3 class=list6_t1Product/h3
p class=list6_text1 mg/ml in Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.2./p
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h3 class=list6_t1Purification amp; Purity/h3
p class=list6_textThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is gt; 95% (by SDS-PAGE)./p
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h3 class=list6_t1Specificity/h3
p class=list6_textp-Cdc25A (S75) antibody detects endogenous levels of p-Cdc25A protein./p
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h3 class=list6_t1Applications (Recommended Dilutions)/h3
p class=list6_textWB: 1:500~1:1000/p
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div id=westernpic
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h3Western Blot(WB)/h3
img id=wpic src=http://www.bioworlde.com/upload/web/4715.jpg width=120 alt= /
div style=width: 250pxWestern blot (WB) analyzes of p-Cdc25A (S75) antibody in extracts from Jurkat UV cells./div
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h3 class=list6_t1Storageamp;Stability/h3
p class=list6_textStore at 4/p
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div class=list6_li
h3 class=list6_t1Background/h3
p class=list6_textThe Cdc2/cyclin B enzyme, involved in regulation of mitosis in eukaryotic cells, is subject to multiple levels of control. Among these, the regulation of the catalytic subunit by tyrosine phosphorylation is the best understood. Tyrosine phosphorylation inhibits the Cdc2/cyclin B complex, while tyrosine dephosphorylation, which occurs at the onset of mitosis, directly activates the pre-MPH complex. The Cdc25 gene serves as a rate-limiting mitotic activator, apparently due to its action as the Cdc2 tyrosine phosphatase. In the absence of Cdc25, Cdc2 accumulates in a tyrosine phosphorylated state. In addition, Cdc25 proteins from a variety of species have been shown to share a low degree of sequence similarity with other tyrosine phosphatases. The Cdc25 gene family consists of at least three members that share approximately 40% identity in their most conserved carboxy terminal sequences./p
/div
/div
div class=list6_li
h3 class=list6_t1Product/h3
p class=list6_text1 mg/ml in Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.2./p
/div
div class=list6_li
h3 class=list6_t1Purification amp; Purity/h3
p class=list6_textThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is gt; 95% (by SDS-PAGE)./p
/div
div class=list6_li
h3 class=list6_t1Specificity/h3
p class=list6_textp-Cdc25A (S75) antibody detects endogenous levels of p-Cdc25A protein./p
/div
div class=list6_li
h3 class=list6_t1Applications (Recommended Dilutions)/h3
p class=list6_textWB: 1:500~1:1000/p
/div
div id=pic
div id=westernpic
div class=imagecontainer
h3Western Blot(WB)/h3
img id=wpic src=http://www.bioworlde.com/upload/web/4715.jpg width=120 alt= /
div style=width: 250pxWestern blot (WB) analyzes of p-Cdc25A (S75) antibody in extracts from Jurkat UV cells./div
/div
/div
/div
div class=list6_li
h3 class=list6_t1Storageamp;Stability/h3
p class=list6_textStore at 4/p
/div
div class=list6_li
h3 class=list6_t1Background/h3
p class=list6_textThe Cdc2/cyclin B enzyme, involved in regulation of mitosis in eukaryotic cells, is subject to multiple levels of control. Among these, the regulation of the catalytic subunit by tyrosine phosphorylation is the best understood. Tyrosine phosphorylation inhibits the Cdc2/cyclin B complex, while tyrosine dephosphorylation, which occurs at the onset of mitosis, directly activates the pre-MPH complex. The Cdc25 gene serves as a rate-limiting mitotic activator, apparently due to its action as the Cdc2 tyrosine phosphatase. In the absence of Cdc25, Cdc2 accumulates in a tyrosine phosphorylated state. In addition, Cdc25 proteins from a variety of species have been shown to share a low degree of sequence similarity with other tyrosine phosphatases. The Cdc25 gene family consists of at least three members that share approximately 40% identity in their most conserved carboxy terminal sequences./p
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