Olomoucine
- 貨號 Cat. No.
- O-300
- 規格 Size
- 10 x 0.1 mg 1mg 5x1mg
Description
Olomoucine
A Cyclin Dependent Kinase (CDK) Inhibitor
| Product#: | O-300 |
| Sizes: |
| 10 x 0.1 mg |
| 1 mg |
| 5 x 1 mg |
is a cell-permeable purine protein kinase inhibitor, found to be highly specific for the cell-cycle regulating p34cdc2/cyclin B, p33cdk2/cyclin A and p33cdk2/cyclin E kinases, the brain p33cdk5/p35 kinase and the ERK1/2 MAP- kinase (IC50=7-25 mM).1
| M.W.: | 298.35 |
| CAS No.: | [1-1622-51-9] |
| Structure: |
 |
| Purity: | >99% |
| Bioassay: |
| Olomoucine (#O-300) prevents NGF-mediated survival of PC12 cells. |
 |
| PC12 cells were grown in the absence of serum. The cells were not protected (fragmented line) or protected from apoptosis with 100 ng/ml mNGF 2.5S (#N-240, continuous line) and treated with different concentrations of Olomoucine. The survival of the cells after 4 days was measured using XTT method, calculated as a relative percentage of the control without Olomoucine and plotted against Olomoucine concentrations. |
| References: |
1. | Vesely, J. et al. (1994) Eur. J. Biochem. 224, 771. |
2. | Glab, N. et al. (1994) FEBS Lett. 353, 207. |
3. | Abraham, R.T. (1995) Biol. Cell 83, 105. |
4. | Schutte, B. et al. (1997) Exp. Cell Res. 236, 4. |
5. | Meijer, L. et al. (1997) Eur. J. Biochem. 243, 527. |
6. | Flament, S. (2000) Zygote 8, 3. |
7. | Rossi, L.A. et al. (1998) Exp. Cell. Res. 245, 8. |
8. | Yakisich, J.S. et al. (1998) Biochem. Biophys. Res. Commun. 243, 674. |
9. | Wesierska-Gadek, J. et al. (2004) Pol. J. Pharmacol. 56, 635. |
10. | Dobashi, Y. et al. (2000) J. Biol. Chem. 275, 12572. |
11. | Iseki, H. et al. (1997) Surgery 122, 187. |
12. | Monaco, E.A. et al. (2004) Biochem. Pharmacol. 67, 1947. |
13. | Ohnuma, S. et al. (2001) Curr. Opin. Neurobiol. 11, 66. |
OlomoucineOlomoucine behaves as a competitive inhibitor for ATP and as a non-competitive inhibitor for histone H1 binding in CDKs kinases.1-5
Olomoucine induces a dose-dependent inhibition of proliferation rate and the cell cycle progression in many human cancer cells in culture (EC50=40-90mM). Olomoucine, at 1-100 mM, slowdown or arrest KB 3-1 cells in G1/S and G2/M phase and decreased the DNA synthesis of rat brain cortex.6-11
Olomoucine also induces apoptosis in maturing cerebellar granule neurons,11 NGF induced neuronal differentiation of PC12 cells in vitro12 and in NE cells in vivo.13