Oligomycin A

Oligomycin A inhibits membrane bound mitochondrial ATP synthase (ATPase), proton channel (pump, FO subunit) blockers to the cell membrane. Oligomycin A also inhibits oxidative phosphorylation and the electron transport chain. Oligomycin A decreased cytoplasmic ATP contents and disassembled metaphase II oocyte spindles when introduced to mouse oocytes. Oligomycin A is used to prevent state 3 (phosphorylating) respiration in oxidative phosphorylation research.