Oligomeric Human beta-Amyloid Abeta1-42 Peptide, Stabilized
Proteins and Peptides
Description
ZZZZ_Status (Active, Disc) : Active
AA_Supplier Name : Biosensis
Alternative_Name (Other Names) :
Beta-APP42; Beta-amyloid protein 42; ABPP; APPI; Amyloid beta A4 protein; AB42; abeta
Antibody_Isotype
(Antibody only) :
Application_Details :
Use as positive control in Oligomeric Aβ ELISA Kit (BEK-2215): Reconstitute one vial with 1 mL of assay buffer provided in the ELISA kit. Dilute to a concentration of 0.5-1 ng/mL. At this concentration, a positive signal will be obtained within the dynamic range of the calibration curve.
Use as oligomeric A-beta peptide standard in Oligomeric Aβ ELISA Kit (BEK-2215): Reconstitute one vial with 1 mL of assay buffer provided in the ELISA kit. Dilute to a concentration of 2 ng/mL, which represents the highest concentration of the calibration curve. Perform a 1:2 serial dilution down to 0.031 ng/mL in assay buffer. Click here for detailed instructions on generating a calibration curve with PE-1750-1000.
Use as positive control in other applications: Optimal concentrations need to be determined empirically. It is recommended to reconstitute the vial with 100 €“ 200 uL buffer first (eg., PBS, pH 7.4), and prepare further working dilutions thereof.
Background_Info :
Clone_Name
(AB only, monoclonals) :
Datasheet_Link : http://www.biosensis.com/pdf_datasheet.php?products_id=1571
Description (Long Description) :
A proprietary preparation of human amyloid beta peptide (amino acids 1-42) that was initially monomerized by HFIP-treatment and then allowed to form oligomers by the procedure described in Youmans KL et al., 2012, followed by lyophilisation using Biosensis€™ proprietary stabilization procedures.
The resulting oligomeric mixture has been specially designed to allow the formation of stable, oligomeric Aβ1-42 peptide, multimeric complexes or oligomers. The material is intended to be used as a stable and consistent standard or positive control for oligomeric ELISA assays, as well as other research applications.
Expiry_Date :
Stability of unopened vials is 6 months if stored appropriately.
Format (Form) :
Lyophilized, Supplied as 2 x 500 ng vials, each containing lyophilized Aβ oligomers. Note that the amount of provided oligomeric protein is based on the amount of monomeric Aβ used to form these oligomers. The precise formation, size and number of oligomers cannot be quantified by any known method.
Immunogen (Antigen) :
Kit_Components :
MSDS (File name) :
