Oligomeric Amyloid-beta ELISA Kit (2 plates)
Kits
- 貨號 Cat. No.
- BEK-2215-2P
- 規格 Size
- 2 plates
- 品牌 Brand
- Biosensis
- Application 應用
- ELISA
- Reactivity
- Rat
- Clonality
- Monoclonal-Monoclonal
Description
ZZZZ_Status (Active, Disc) : Active
AA_Supplier Name : Biosensis
Alternative_Name (Other Names) :
Beta-APP42; Beta-APP40; Beta-amyloid protein 42; Beta-amyloid protein 40; ABPP; APPI; Amyloid beta A4 protein; MOAB2; MOAB-2; Alzheimer's antibody; AB40; AB42; abeta.
Antibody_Isotype
(Antibody only) :
Application_Details :
Please download the detailed product insert for complete instructions for the successful use of this ELISA; Use only as directed.
Background_Info :
Clone_Name
(AB only, monoclonals) :
Datasheet_Link : http://www.biosensis.com/pdf_datasheet.php?products_id=1286
Description (Long Description) :
The oligomeric form of Amyloid Beta peptide (Aβ, 1-42) has been closely linked to Alzheimer€™s Disease. Several ELISAs targeting Aβ have been developed; however, these ELISAs are known to cross-react with Amyloid Beta precursor protein (APP) and are poorly characterized against monomeric and oligomeric forms of the peptide. The Biosensis MOAB-2 antibody, developed by LaDu and co-workers (Youmans K. et al., 2012), has been shown to specifically detect Aβ, but not the precursor molecule APP. When utilized in ELISAs, the oligomeric form of Aβ peptide (o-Aβ) can be assayed independently of the other forms of the molecule when assayed with the MOAB-2 monoclonal antibody.
The Biosensis oligomeric Aβ ELISA kit is a sandwich ELISA that allows the preferential quantification of oligomeric Aβ peptides. This kit is exclusive to Biosensis and consists of a pre-coated mouse monoclonal anti-Aβ capture antibody (MOAB-2), a biotinylated MOAB-2 detection antibody and horseradish peroxidase (HRP)-conjugated streptavidin. The addition of a substrate (3,3€™,5,5€™-tetramethylbenzidine, TMB) yields a colored reaction product which is directly proportional to the concentration of o-Aβ present in samples and protein standards. The purpose of this kit is the in vitro qualitative measurement of oligomeric Aβ peptide levels in brain extracts and CSF samples from both transgenic mice and humans relative to a known o-Aβ standard. The inclusion of a highly validated oligomeric standard results in a unique, ready-to-use ELISA kit.
This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
Expiry_Date :
12 months from purchase.
Format (Form) :
Ready to use Assay, complete with pre-coated plates, Concentrated Buffers, Detection reagents and complete instructions.
Immunogen (Antigen) :The standard in this ELISA is synthetically manufactured beta-amyloid peptide, amino acids 1-42 of human, HFIP treated and dried.The stabilized oligomeric beta amyloid 1-42 control complex is also constructed from the same synthetic peptide standard material. No animal systems were used for their manufacture.
Kit_Components : The ELISA kit box contains 2 x 96-well pre-coated strip plates, protein standards, QC sample, detection reagents, wash and sample buffers, substrate buffer and detailed protocols.
MSDS (File name) :
