Okadaic Acid
- 貨號 Cat. No.
- O-900
- 規格 Size
- 10x5 μg 25 μg 5x25 μg 100 μg
Description
Okadaic Acid
Sodium Salt
Potent, Inhibitor of PP-1 and PP-2A, Protein Phosphatases
| Product#: | O-900 |
| Sizes: |
| 10 x 5 mg |
| 25 mg |
| 5 x 25 mg |
| 100 mg |
| M.W.: | 827 |
| CAS No.: | 209266-80-8 |
| Structure: |
| . |
 |
| Purity: | >98% |
| Bioassay: |
| Okadaic Acid induces apoptosis in Jurkat cells and inhibition of protein phosphatase 2A in 3T3-L1 cells. |
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Jurkat cells were grown to 70% confluency. Then 1 mM Okadaic Acid (#O-900) or vehicle were added for 6 hours. At the end of the incubation period, the cells extracts were detected for cleaved Caspase 3 with specific antibodies.
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| 3T3-L1 cells were grown to confluency then serum starved for 18 h. For in vivo phosphatase activity measurement, the growth medium was replaced to HENKî–˜ and the cells were preincubated for 30 min in the presence or absence of 100 nM Okadaic Acid (#O-800, #O-900) and 100 nM I-Nor-Okadaone (negative control) and for 20 min with or without 20mM NEM. The cells were stimulated with 5 ng/ml PDGF for 30 min and cells extracts were analyzed by Western blotting for Akt using Ser-473 phospho-specific antibodies. |
| References: |
1. | Murakumi, Y et al. (1982) Bull. Jpn. Soc. Sci. Fish. 48, 69. |
2. | Suganuma, M. et al. (1988) PNAS, 85, 1768. |
3. | Cohen, P. et al. (1990) Trends Biochem Sci. 15, 1837. |
4. | Haystead, T.A. et al. (1989) Nature 337, 78081. |
5. | Higuchi, M. and Aggarwal, B.B. (1993) J. Biol. Chem. 268, 5624. |
6. | Murakumi, Y et al. (1982) Bull. Jpn. Soc. Sci. Fish. 48, 69. |
Okadaic acid is a polyether compound, isolated from a black sponge, Halichondria okadai. It is previously demonstrated as a non-TPA-type tumor promoter and then as a cell-permeable, potent and specific inhibitor of serine/threonine protein phosphatases 1 and 2A. In higher concentrations Okadaic acid induced apoptosis (IC50 = 0.5 mM).1-4
Okadaic acid down-regulated the TNF receptor, induces the release of Ca2+ from intracellular stores in ECV304 endothelial cells and inhibits GH4 cell proliferation in a concentration-dependent manner (IC50 = 5 nM).5,6