Oct-4 Antibody

Antibody Target :Oct-4 Polyclonal Ab

Host: Rabbit

Antibody Type: Polyclonal

Isotype: Rabbit IgG

Antibody Form: Affinity Purified Antibody

Immunogen Sequence: Synthetic peptide mapping to the N-terminus of mouse Oct-4

Accession#: P20263

Gene ID: 18999

Appearance: Colorless liquid

Concentration: 0.5 mg/ml

Formulation: 100 µg (0.5 mg/ml) affinity purified rabbit anti-Oct-3/4 polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 0.01% thimerosal.

Purification: Affinity purified

Species Reactivity: hu/ms/rt

Application: WB/IP/IHC

Application & Usage: Western blotting (0.5-4 µg/ml), immunoprecipitation (20 µg/ml) and immunohistochemistry (20 µg/ml). However, the optimal conditions should be determined individually. The antibody reacts with 45 kDa Oct-4 (also designated Oct-3A) and in a less extent 33 kDa Oct-3B from human, mouse, and rat samples. Rat kidney tissue lysate can be used as a positive control.

Storage Temp.: -20 °C

Shipping : gel pack

Shelf Life: 12 months

Handling: The antibody solution should be gently mixed before use.

Brief Background Descriptions: Members of Oct family of transcription factors specifically interact with Octamer motif ATGCAAAT, a regulatory element important for tissue- and cell-specific transcription as well as for transcription of a number of housekeeping genes. All of the members of the Oct family contain two highly conserved domains which are separated by 14-26 variable amino acids. These include the POU homeodomain and the POU-specific domain. Both are required for DNA binding and are involved in protein-protein interactions. Evidences indicate that regulation of Oct family transcription factors occurs at the level of phosphorylation.