Occludin Antibody (LS-B2320)

貨號 Cat. No.: LS-B2320
規格 Size: 50 Âµg
品牌 Brand: LSBio

Description

Antibody Catalog ID:	LS-B2320
Antibody Validation:	This antibody replaces catalog number LS-C37845. It has been validated for use in the following assays: IHC-P.
Antibody Target:	Occludin (OCLN)
Synonyms:	OCLN, occludin
Host:	OCLN antibody was produced in Mouse
Antigen Species:	OCLN antibody was raised against Human
Clonality:	Monoclonal
Isotype:	IgG1,k
Antigen Type:	Fusion protein
Immunogen:	OCLN antibody was raised against gST fusion protein consisting of the C-terminal region (~150aa) of human occludin.
Antibody Specificity:	Recognizes mammalian Occludin Protein. Species cross-reactivity: human, mouse, rat and canine.
Antigen Modification:	C-Terminus
Predicted Species Reactivity:	Dog, Mouse, Rat
Purification:	Protein A Column
Presentation:	PBS, pH 7.4, 0.1% sodium azide.
Recommended Storage:	+4°C or -20°C, Avoid repeated freezing and thawing.
Antibody Usage Summary:	Immunohistochemistry: LS-B2320 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody LS-D2, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2320 was determined to be 10 ug/ml. Positive control: T84 cell line (human intestinal epithelium), MDCK cells (canine kidney), rat liver and Caco-2 cells (human colon adenocarcinoma). Does not appear to detect the most highly phosphorylated form of the occludin protein when tested on Caco-2 cells.
Antibody Uses:	Immunohistochemistry - Paraffin (IHC-P) (10 µg/ml), Western blot (WB) (0.1 - 1 µg/ml), Immunofluorescence (IF) (2 - 3 µg/ml), ELISA (0.1 - 1 µg/ml) (Optimal dilution to be determined by the researcher)
Size:	50 µg
Concentration:	0.5 mg/ml