Occludin Antibody (LS-B2187)

貨號 Cat. No.: LS-B2187
規格 Size: 50 Âµg
品牌 Brand: LSBio

Description

Antibody Catalog ID:	LS-B2187
Antibody Validation:	This antibody replaces catalog number LS-C37847. It has been validated for use in the following assays: IHC-P.
Antibody Target:	Occludin (OCLN)
Synonyms:	OCLN, occludin
Host:	OCLN antibody was produced in Rabbit
Antigen Species:	OCLN antibody was raised against Human
Clonality:	Polyclonal
Isotype:	IgG
Antigen Type:	Fusion protein
Immunogen:	OCLN antibody was raised against a GST fusion protein consisting of the C-terminal 150 amino acids of human occludin fused to GST.
Antibody Specificity:	Recognizes mammalian occludin. Reactivity of this antibody with the occludin protein has been confirmed by Western Blot using total cell lysates derived from MDCK cells, human T84 cells, and rat fibroblasts transfected with a plasmid encoding human occludin. Species cross-reactivity: Human, mouse, rat and canine.
Antigen Modification:	C-Terminus
Predicted Species Reactivity:	Dog, Mammal, Mouse, Rat
Purification:	Immunoaffinity Chromatography
Presentation:	PBS, pH 7.4, 0.1% sodium azide.
Recommended Storage:	Long term: -20°C; Short term: +4°C
Antibody Usage Summary:	Immunohistochemistry: LS-B2187 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody LS-D1, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2187 was determined to be 2.5 ug/ml.
Antibody Uses:	Immunohistochemistry - Paraffin (IHC-P) (2.5 µg/ml), Western blot (WB) (1 - 3 µg/ml), Immunoprecipitation (IP), Immunofluorescence (IF), ELISA (0.1 - 1 µg/ml) (Optimal dilution to be determined by the researcher)
Size:	50 µg
Concentration:	0.25 mg/ml