Proteins and Peptides
NUMB Antibody (LS-B265)
- 貨號 Cat. No.
- LS-B265
- 規格 Size
- 50 µl
- 品牌 Brand
- LSBio
Description
| Antibody Catalog ID: | LS-B265 |
|---|---|
| Antibody Validation: | This antibody replaces catalog number LS-C2586. It has been validated for use in the following assays: IHC. |
| Antibody Target: | Numb Homolog (NUMB) |
| Synonyms: | NUMB |
| Host: | NUMB antibody was produced in Rabbit |
| Antigen Species: | NUMB antibody was raised against Mouse |
| Clonality: | Polyclonal |
| Antigen Type: | Synthetic peptide - KLH conjugated |
| Immunogen: | NUMB antibody was raised against synthetic peptide |
| Antibody Specificity: | A synthetic peptide made to a C-terminal region of the murine numb protein sequence (between residues 600-653). |
| Antigen Modification: | aa600-653 |
| Predicted Species Reactivity: | Human, Rat |
| Purification: | Affinity Chromatography |
| Presentation: | Tris-glycine buffer, 150 mM sodium chloride, 0.05% sodium azide. |
| Recommended Storage: | Long term: -70°C; Short term: +4°C |
| Antibody Usage Summary: | Immunohistochemistry: LS-B265 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody LS-D1, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B265 was determined to be 2.5 ug/ml. |
| Antibody Uses: | Immunohistochemistry - Paraffin (IHC-P) (2.5 µg/ml), Western blot (WB) (0.2 - 0.5 µg/ml), Immunoprecipitation (IP) (Optimal dilution to be determined by the researcher) |
| Size: | 50 µl |
| Concentration: | 3.5 mg/ml |
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