Proteins and Peptides
NTRK2 / TRKB Antibody (LS-B2544)
- 貨號 Cat. No.
- LS-B2544
- 規格 Size
- 50 µg
- 品牌 Brand
- LSBio
Description
| Antibody Catalog ID: | LS-B2544 |
|---|---|
| Antibody Validation: | This antibody replaces catalog number LS-C18197. It has been validated for use in the following assays: IHC-P. |
| Antibody Target: | NTRK2 / TRKB |
| Synonyms: | NTRK2 Antibody, gp145-trkb Antibody, neurotrophic tyrosine kinase receptor type 2 Antibody, ntrk2 Antibody, trk-B Antibody, trkb Antibody, TrkB Neurotrophin Receptor Antibody, trkb tyrosine kinase Antibody, Tyrosine kinase receptor b Antibody |
| Family: | Protein Kinase |
| Subfamily: | Trk/Ror |
| Host: | NTRK2 antibody was produced in Rabbit |
| Antigen Species: | NTRK2 antibody was raised against Rat |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Antigen Type: | Cells |
| Immunogen: | NTRK2 antibody was raised against the entire extracellular domain (corresponding to residues 1-429) of the rat TrkB receptor, expressed in COS cells. |
| Antibody Specificity: | Recognizes rat TrkB, Mr 145kD, as well as an additional protein, Mr ~90kD, which is likely a TrkB degradation product. Species sequence homology: mouse 97% and human 88%. |
| Antigen Modification: | aa1-429 |
| Predicted Species Reactivity: | Mouse |
| Purification: | Protein A Column |
| Presentation: | PBS, pH 7.6, 0.07% sodium azide, 30% glycerol. |
| Recommended Storage: | +4°C or -20°C, Avoid repeated freezing and thawing. |
| Antibody Usage Summary: | Immunohistochemistry: LS-B2544 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody LS-D1, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2544 was determined to be 10 ug/ml. Western Blot: 0.5-2 ug/ml detected TrkB in mouse brain membrane protein preparations. |
| Antibody Uses: | Immunohistochemistry - Paraffin (IHC-P) (10 µg/ml), Western blot (WB) (0.5 - 2 µg/ml) (Optimal dilution to be determined by the researcher) |
| Size: | 50 µg |
| Concentration: | 1 mg/ml |
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