NOS Detection Kit

Diaminofluorescein-2 diacetate (DAF-2DA) is a non-fluorescent cell permeable reagent that can measure free nitric oxide (NO) and nitric oxide synthase (NOS) activity in living cells under physiological conditions (cell culture or tissue sections) in real time. Once inside the cell the diacetate groups of DFA-2DA are hydrolyzed by cytosolic esterases thus releasing DAF-2 and sequestering the reagent inside the cell. Production of nitric oxide converts the non-fluorescent dye, DAF-2, to its fluorescent triazole derivative, DAF-2T (excitation at 488 nm and emission at 515 nm). The one step no-wash assay is adaptable for HTS format. The kit is available with 125 mg or 250 mg DFA-2DA reagent. Application: Fluorescence.

Not for sale in Japan.