nNOS Antibody

Product Usage Information

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

nNOS Antibody detects endogenous levels of total nNOS protein.

Species Reactivity:

Human, Mouse, Rat

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to human nNOS. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Nitric Oxide Synthase (NOS) catalyzes the formation of nitric oxide (NO) and citruline from L-arginine, oxygen and cofactors. Three family members have been characterized: neuronal NOS (nNOS), which is found primarily in neuronal tissue; inducible NOS (iNOS), which is induced by interferon gamma and lipopolysaccharides in the kidney and cardiovascular system; and endothelial NOS (eNOS), which is expressed in blood vessels (1). NO is a messenger molecule with diverse functions throughout the body including the maintenance of vascular integrity, homeostasis, synaptic plasticity, long-term potentiation, learning, and memory (2,3).

nNOS binds selectively to the second PDZ domain of PSD-95, and the enzymatic activity of nNOS is either downregulated by phosphorylation at Ser847 by CaM-KII or stimulated by calcium influxes governed by the NMDA receptor channels (4-5).

1. Tsutsui, M. (2004) J Atheroscler Thromb 11, 41-48.


2. Son, H. et al. (1996) Cell 87, 1015-23.


3. Hawkins, R.D. (1996) Neuron 16, 465-467.


4. Watanabe, Y. et al. (2003) Biochem J 372, 465-71.


5. Sears, C.E. et al. (2004) Philos Trans R Soc Lond B Biol Sci 359, 1021-44.