NK-92
- 貨號 Cat. No.
- CLC027
- 品牌 Brand
- gene-rl
Description
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Cell Lines (GRL-CLC027) | |
Designations | NK-92 |
| Cell type | Human natural killer cell |
Growth property | Suspension |
| Seeding density(cells/ml) | 2X106 |
Survival rate | 95% |
Culture medium | 75% Alpha minimum essential medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate with 0.2 mM inositol, 0.1 mM 2-mercaptoethanol, 0.02 mM folic acid, 100-200 U/ml recombinant IL-2 + 12.5% horse serum + 12.5% fetal bovine serum |
Incubation condition | 37°C，5% CO2 |
Freezed medium | 90% culture medium + 10% DMSO |
Medium renewal | every 2 to 3 days |
Subculturing |
Cultures can be maintained by addition or replacement of fresh medium. |
Mycoplasma test | Negative for bacteria，fungi and mycoplasma |
Advertence: |
When you receive the cell line, please examine the flask and its cap. If it is not well-found, please tell us instantly. After examination, please wipe the flask and flask cap with 70% alcohol, and then loosen it. Afterward, put the flask into incubator immediately.