Nitric Oxide Colorimetric Assay Kit
Kits
- 貨號 Cat. No.
- K262-200
- 規格 Size
- K262-200
- Sample Type
- as well as many other biological fluids 、 Cell and tissue lysates 、 culture media 、 Plasma and Serum 、 urine
Description
Alternate Name :
Tag Line :
Detects total nitrate/nitrite
Storage Conditions: -20°C
Shelf life: 12 months
Summary :
• Detection method- Absorbance (540 nm)
• Species reactivity- Mammalian
• Applications- BioVision’s Nitric Oxide Colorimetric Assay Kit provides an accurate, convenient measure of total nitrate/nitrite in a simple two-step process.
Detection Method:
Features & Benefits:
• Simple two-step procedure; takes only ~1-1.5 hours
• Fast and convenient
• BioVision’s Nitric Oxide Colorimetric Assay Kit provides an accurate, convenient measure of total nitrate/nitrite.
Description:
Nitric oxide (NO) plays an important role in neurotransmission, vascular regulation, immune response and apoptosis. NO is rapidly oxidized to nitrite and nitrate which are used to quantitate NO production. BioVision’s Nitric Oxide Colorimetric Assay Kit provides an accurate, convenient measure of total nitrate/nitrite in a simple two-step process. The first step converts nitrate to nitrite utilizing nitrate reductase. The second step uses Griess Reagents to convert nitrite to a deep purple azo compound. The amount of the azochromophore accurately reflects nitric oxide amount in samples. The detection limit of the assay is approximately 0.1 nmole nitrite/well, or 1 µM.