NGFR/p75ECD Rapid ELISA Kit: Human (2 Plates)

ZZZZ_Status (Active, Disc) : Active

 

AA_Supplier Name : Biosensis

 

Alternative_Name (Other Names) :

Low-affinity nerve growth factor receptor; NGF receptor; Gp80-LNGFR; p75 ICD; Low affinity neurotrophin receptor p75NTR; Tumor necrosis factor receptor superfamily member 16; CD271; NGFR; TNFRSF16.

 

Antibody_Isotype
(Antibody only) :

 

Application_Details :

Please download the detailed product insert for complete instructions for the successful use of this ELISA; Use only as directed.

 

Background_Info : The nerve growth factor (NGF) receptor (NGFR), also known as p75 neurotrophin receptor (p75NTR; TNFRS16; CD271) is a common receptor for the neurotrophins NGF, BDNF, NT-3 and NT-4/5. In neurons, p75NTR mediates a variety of physiological functions including survival, apoptosis, neurite outgrowth and synaptic plasticity. A potential pathological role for p75NTR has become evident in recent years. Altered p75NTR expression levels are implicated in degeneration of spinal motor neurons in human and mouse models of amyotrophic lateral sclerosis (ALS). Importantly, the extracellular domain of p75NTR (p75ECD) is shed from the cell membrane and excreted in urine. Recent findings further suggest that p75ECD could be an early biomarker for ALS in humans, as significantly elevated p75ECD levels are found in urine of ALS patients as compared to healthy controls.

 

Clone_Name
(AB only, monoclonals) :

 

Datasheet_Link : http://www.biosensis.com/pdf_datasheet.php?products_id=1719

 

Description (Long Description) : 

The Biosensis NGFR/p75^ECD Rapid^TM enzyme-linked immunosorbent assay (ELISA) Kit is a sandwich ELISA that allows the quantification of the extracellular domain (ECD) of human p75 neurotrophin receptor in less than 4 hours in urine, serum, citrate-plasma, CSF and cell culture supernatants only if used as directed. Please refer to the kit protocol for specific use instructions for each substrate application.

Note that capture and detection antibodies have been optimized for increased assay sensitivity, and reagents from previous lots should not be used. This optimized human p75^ECD ELISA assay demonstrates an increased sensitivity, lowering the LOD about 8-fold to the previous assay version. It represents the most sensitive NGFR/p75^ECD ELISA assay available today, with a detection limit of at least 6 pg/mL, and a quantification range of 7.8 - 500 pg/mL.

This ELISA kit consists of a pre-coated mouse monoclonal anti-p75^ECD capture antibody, a biotinylated mouse monoclonal anti-p75^ECD detection antibody and horseradish peroxidase (HRP)-conjugated streptavidin. The addition of a substrate (3,3€™,5,5€™-tetramethylbenzidine, TMB) yields a colored reaction product which is directly proportional to the concentration of p75^ECD present in samples and protein standards. A human p75^ECD positive control (QC sample) is provided to assure consistent assay performance.

This NGFR/p75^ECD ELISA kit employs an in-house recombinant human p75^ECD-Fc chimera as standard that has been validated against an externally available p75^ECD-Fc protein. While there is a current lack of a commercially available, true human p75^ECD standard, this ELISA kit allows quantification of human p75^ECD as p75^ECD-Fc human equivalents. Please note that the antibodies used in this ELISA do not cross-react with mouse p75^ECD.

Note: For research use only. Not for diagnostic and clinical purposes.

The nerve growth factor (NGF) receptor (NGFR), also known as p75 neurotrophin receptor (p75NTR; TNFRS16; CD271) is a common receptor for the neurotrophins NGF, BDNF, NT-3 and NT-4/5. In neurons, p75NTR mediates a variety of physiological functions including survival, apoptosis, neurite outgrowth and synaptic plasticity. A potential pathological role for p75NTR has become evident in recent years. Altered p75NTR expression levels are implicated in degeneration of spinal motor neurons in human and mouse models of amyotrophic lateral sclerosis (ALS). Importantly, the extracellular domain of p75NTR (p75ECD) is shed from the cell membrane and excreted in urine. Recent findings further suggest that p75ECD could be an early biomarker for ALS in humans, as significantly elevated p75ECD levels are found in urine of ALS patients as compared to healthy controls.

 

Expiry_Date : 

12 months from purchase.

 

Format (Form) : 

Ready to use Assay, complete with pre-coated plates, Concentrated Buffers, Detection reagents and complete instructions.

 

Immunogen (Antigen) :Recombinant human p75 (AA 1-250) -Fc chimera, made in 293 cells

 

Kit_Components : The ELISA kit box contains 2 x 96-well pre-coated strip plates, protein standards, QC sample, detection reagents, heterophilic antibody blocker, wash and sample buffers, substrate buffer and detailed protocols.

 

MSDS (File name) :MSDS_Rapid_ELISA_as_at_Aug2017.pdf