NGAL (Rat) ELISA

pNGAL (Rat) ELISA/p
pimg alt=For Research Use Only src=http://www.alpco.com/img/ruo.gif align=absMiddle / For Research Use Only/p
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div class=prod_specbFormat:/b/div
div class=prod_spec_infoELISA / EIA/div
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div class=prod_specSample Type:/div
div class=prod_spec_infoserum, plasma, cell culture, tissue culture, urine/div
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div class=prod_specbSample Size:/b/div
div class=prod_spec_info10 micro;l/div
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div class=prod_specbSize:/b/div
div class=prod_spec_info96 wells/div
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div class=prod_specbRange:/b/div
div class=prod_spec_info0.5 - 5.0 U/ml/div
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div class=prod_specbSensitivity:/b/div
div class=prod_spec_info0.009 U/ml/div
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div class=prod_specbIncubation Time:/b/div
div class=prod_spec_info3 hour(s) 10 min(s)/div
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pbSummary:/b/p
NGAL (neutrophil gelatinase-associated lipocalin) belongs to the lipocalin family of proteins. These are secreted proteins characterized by i) their ability to bind small hydrophobic molecules in a structurally conserved pocket formed by beta;-pleated sheet, ii) to bind to specific cell-surface receptors, and iii) to form macromolecular complexes. NGAL has many synonyms: perhaps the most widely used is lipocalin 2 (LCN 2); more recently the name siderocalin has been used to express NGALrsquo;s ability to bind bacterial siderophores. In the rat, NGAL was first named 25 kDa alpha;2-microglobulin-related protein,4 and later neurelated lipocalin (NRL)5 because of its expression in mammary tumors initiated by the neu (HER2/c-erbB-2) oncogene. In the mouse, the homologous protein is known as oncogene protein 24p3, 24-kDa superinducible protein (SIP24) or uterocalin.br /
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Rat NGAL consists of a single disulfide-bridged polypeptide chain of 178 amino-acid residues with a calculated molecular mass of 20.5 kDa, but glycosylation increases its apparent molecular mass to 25 kDa. In some situations NGAL may be co-expressed and form complexes with matrix metalloproteinase-9 (MMP-9; also called gelatinase B in human neutrophils, hence NGALrsquo;s name). In the adult rat, NGAL is expressed in the liver, prostate, kidney, mammary gland and epithelial cells of the respiratory and alimentary tracts. It may be expressed in additional cell types during embryonic development and in response to inflammatory mediators. NGAL expression in hepatocytes is increased by exposure to IL-1beta; but not to IL-6. Molecular forms of rat NGAL have been studied in vascular smooth muscle cells. In these cells, NGAL and MMP-9 are co-synthesized in response to angioplastic injury or stimulation with IL-1beta;. Rat NGAL was shown by immunoblotting to occur in monomer, dimer and trimer forms, as well as a 150-kDa form. MMP-9 occurred in monomer and dimer forms. While complexes of rat NGAL and MMP-9 could not be demonstrated in lysed intimal smooth muscle cells, NGAL/MMP-9 complexes of approximately 115 and 125 kDa were detected in the culture medium, demonstrating the extracellular but not intracellular formation of NGAL/MMP-9 complexes. Rat NGAL has no cysteinyl residue additional to the two forming the intra-chain disulfide bridge, so any participation of NGAL cysteinyl residues in self-association or complex formation with MMP-9 is a matter for further study.br /
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The NGAL (Rat) ELISA is manufactured by BioPorto Diagnostics and intended for the determination of NGAL in rat urine, plasma, serum, tissue extracts, or culture media./div
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