NFkB p65 Antibody

Antibody Target :NFkB p65

Host: Mouse

Antibody Type: Monoclonal

Isotype: Mouse IgG1

Antibody Form: Affinity Purified Antibody

Immunogen Sequence: Synthetic peptide from human NFkB

Accession#: NP_033071

Gene ID: 19697

Appearance: Colorless liquid

Concentration: 0.2 mg/ml

Formulation: 100 µg (200 µg/ml) in PBS containing 1 mg/ml BSA and 1.5 mM sodium azide and 50% glycerol.

Purification: Affinity purified

Species Reactivity: hu/ms/rt

Application: WB/IP/IHC

Application & Usage: Western blot analysis (1-4 µg/ml). However, the optimal conditions should be determined individually. A431 cells can be used as a positive control.

Storage Temp.: -20 °C

Shipping : gel pack

Shelf Life: 12 months

Handling: The antibody solution should be gently mixed before use.

Brief Background Descriptions: NF?B was identified as a sequence specific transcriptional activator that binds to the intronic enhancer of kappa light chain gene in B lymphocytes. NF?B is a heterodimer that consists of a 50 kDa DNA binding subunit (p50) and a 65 kDa transactivation subunit (p65/RelA). Both of these subunits exhibit sequence homology to the protooncogene c-Rel. The p50/p65 heterodimer remains in the cytosol in an inactive form as a complex with its inhibitor, I?B. Upon stimulation of cells by a wide variety of stimuli such as lipopolysaccharide (LPS), pro-inflammatory cytokines, and viral infection. I?B is phosphorylated and degraded by proteosome. The active NF?B heterodimer is translocated into the nucleus and induces gene expression