NFkB p50 Polyclonal Antibody

HOST: Rabbit

IMMUNOGEN: Synthetic peptide mapping to the N-terminus of human NF?B p50.

SPECIES REACTIVITY: human, mouse, rat

FORMULATION: 100 ug (0.5 mg/ml) rabbit anti-NF?B p50 polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 0.01% thimerosal.

STORAGE CONDITIONS: Store at -20oC. For long-term storage, aliquot and freeze at -70oC. Avoid repeated freeze/defrost cycles.

BACKGROUND: Nuclear factor kappa B (NF?B) was identified as a sequence specific transcriptional activator that binds to the intronic enhancer of kappa light chain gene in B lymphocytes. NF?B is a heterodimer that consists of a 50 kDa DNA binding subunit (p50) and a 65 kDa transactivation subunit (p65/RelA). Both of these subunits exhibit sequence homology to the protooncogene c-Rel. The p50 has an isoform called p49/p52, and both proteins are derived from the amino-terminal of precursor protein p105 and p100. The p50/p65 heterodimer remains in the cytosol in an inactive form as a complex with its inhibitor, I?B. Upon stimulation of cells by a wide variety of stimuli such as lipopolysaccharide (LPS), pro-inflammatory cytokines (IL-1 & TNF, etc.), and viral infection, I?B is phosphorylated and degraded by proteosome. The active NF?B heterodimer is translocated into the nucleus and induces gene expression. APPLICATIONS: The antibody can be used for Western blotting (0.5-4 ug/ml). However, the optimal conditions should be determined individually.