Neutral endopeptidase

SPECIFICATIONS.

Chromatographically purified, lyophilized, water soluble powder.

MW approximately 95,000

Store at -20°C. Will remain stable for 6 to 8 months at -20°C.

850-900 units per mg of protein.

One unit will liberate 1 µmole of pNA/min/mg protein from Glutaryl-(Ala)₂-Phe-pNA (EPC No. GR511), at pH 7.4 and

25°C.

PREPARATION.

Prepared by the modifications of the method of Gafford, J. (58).

Neutral endopeptidase (NEP; also known as kidney microvillus proteinase, neprilysin, enkephalinase, CD10 and CALLA [common acute lymphocytic leukemia antigen]) is a cell-surface, zinc-containing metallopeptidase that is present in many mammalian tissues including brain, liver, lung and endothelial cells. It is particularly abundant on the brush-border membranes of the kidney proximal tubule. NEP is a peptidase which has a specificity directed toward cleavage on the amino side of hydrophobic residues. In addition, an active site arginine on the enzyme can interact with the C-terminal carboxylate of a substrate.

Greater than 98% purity by HPLC (reverse phase