Neurotrophin Antibody Kit
Antibodies
- 貨號 Cat. No.
- KIT-NT-500
- 規格 Size
- 5 vials, each containing 500 µg sheep IgG
- 品牌 Brand
- Biosensis
- Application 應用
- Neut
- Reactivity
- Human 、 Rodent
- Host
- Sheep
- Clonality
- Polyclonal
Description
ZZZZ_Status (Active, Disc) : Active
AA_Supplier Name : Biosensis
Alternative_Name (Other Names) :
Antibody_Isotype
(Antibody only) : IgG
Application_Details :
Immunohistochemistry: 1-10 µg/mL. BDNF antibody works best on Zamboni's fixed, frozen tissue, and is not recommended for paraffin-embedded tissue.
ELISA: 1-10 µg/mL.
Western Blotting: 1-10 µg/mL. Western blotting is not recommended for the BDNF antibody.
Inhibition of biological activity in vitro/in vivo: Expected ED50 (in vivo) values are in the range of 2-10 µg/mL. The NGF antibody completely inhibits neuronal survival and the outgrowth actions of murine NGF in chicken DRG in vitro.
Biosensis recommends optimal dilutions/concentrations should be determined by the end user. The negative control (normal sheep IgG) should be used at the same concentration as the neurotrophin antibodies.
Background_Info :
Clone_Name
(AB only, monoclonals) :
Datasheet_Link : http://www.biosensis.com/pdf_datasheet.php?products_id=1587
Description (Long Description) :
Neurotrophins are growth factors that regulate neuronal development, maintenance and plasticity. These homo-dimeric proteins activate a multitude of intracellular signalling cascades through their interaction with Trk receptors and p75NTR.
The Neurotrophin Antibody Kit (Trial Size) contains a collection of Biosensis€™ popular sheep antibodies raised against BDNF (S-015-500), NGF (S-052-500), NT3 (S-057-500) and NT4/5 (S-059-500). The kit is completed with one vial of normal sheep IgG (S-1754-100) for use as negative control. This full size version of Biosensis€™ Neurotrophin Antibody Kit contains 500 µg sheep IgG of each antibody.
This kit presents a cost-effective way to investigate neurotrophin protein expression and function by immunological techniques such as immunohistochemistry, ELISA, and inhibition of biological activity.
Expiry_Date :
12 months after purchase (unopened vials).
Format (Form) :
Lyophilized from PBS, pH 7.4, without preservatives.
Immunogen (Antigen) :BDNF: recombinant human BDNF
NGF: native mouse beta NGF purified from submaxillary salivary gland (95% purity by PAGE)
NT3: recombinant human NT3
NT4/5: recombinant human NT4
Kit_Components :
MSDS (File name) :
