Neurotrophin 4/5 (NT4/5) Rapid ELISA Kit: Human, Mouse, Rat and Monkey (2 Plates)

ZZZZ_Status (Active, Disc) : Active

 

AA_Supplier Name : Biosensis

 

Alternative_Name (Other Names) :

Neurotrophin-5; NT-5; Neutrophic factor 5; Neurotrophin-4; NT-4; Neutrophic factor 4; NTF5; NTF4

 

Antibody_Isotype
(Antibody only) :

 

Application_Details :

Please download the detailed product insert for complete instructions for the successful use of this ELISA; Use only as directed.

 

Background_Info : FUNCTION: Target-derived survival factor for peripheral sensory sympathetic neurons. SUBCELLULAR LOCATION: Secreted protein. TISSUE SPECIFICITY: Highest levels in prostate, lower levels in thymus, placenta, and skeletal muscle. Expressed in embryonic and adult tissues. SIMILARITY: Belongs to the NGF-beta family.

 

Clone_Name
(AB only, monoclonals) :

 

Datasheet_Link : http://www.biosensis.com/pdf_datasheet.php?products_id=1296

 

Description (Long Description) : 

The Biosensis Neurotrophin 4/5 Rapid^TM enzyme-linked immunosorbent assay (ELISA) Kit is a sandwich ELISA that allows the specific, fast and reliable quantification of NT4/5 in less than 4 hours in cell culture supernatants, human citrate-plasma and brain extracts only if used as directed. Please refer to the kit protocol for specific use instructions for each substrate application, in particular blood samples. Accurate quantification of NT4/5 in human citrate-plasma requires a heterophilic antibody (HA) blocker which can be purchased separately (BL-003-1000).

This ELISA kit consists of a pre-coated polyclonal anti-NT4/5 capture antibody, a biotinylated anti-NT4/5 detection antibody and horseradish peroxidase (HRP)-conjugated streptavidin. The addition of a substrate (3,3€™,5,5€™-tetramethylbenzidine, TMB) yields a colored reaction product, which is directly proportional to the concentration of NT4/5 present in samples and protein standards.

This NT4/5 ELISA kit employs a recombinant human NT4/5 standard. The capture and detection antibodies will also detect NT4/5 from other species due to a high degree of NT4/5 amino acid sequence homology. Therefore, this ELISA kit can be used to quantify NT4/5 in many species including mouse, rat and monkey.

The antibodies used in this kit bind to epitopes within the mature domain of NT4/5. Thus, this ELISA kit will quantify the mature form of NT4/5, and may also detect the full-length pro-form of NT4/5.

This ELISA kit has not been tested for other applications. It has been configured for research use only and is not to be used for diagnostic or clinical procedures.

 

Expiry_Date : 

12 months from purchase.

 

Format (Form) : 

Ready to use Assay, complete with pre-coated plates, Concentrated Buffers, Detection reagents and complete instructions.

 

Immunogen (Antigen) :Recombinant human NT4/5, made in E.coli

 

Kit_Components : The ELISA kit box contains 2 x 96-well pre-coated strip plates, protein standards, detection reagents, wash and sample buffers, substrate buffer and detailed protocols.

 

MSDS (File name) :