Neurotrophin 3 (NT3) Rapid ELISA Kit: Human, Rat and Mouse (2 Plates)

ZZZZ_Status (Active, Disc) : Active

 

AA_Supplier Name : Biosensis

 

Alternative_Name (Other Names) :

Neurotrophin-3; Neurotrophic factor; HDNF; Nerve growth factor 2; NGF-2; Ntf3; Ntf-3

 

Antibody_Isotype
(Antibody only) :

 

Application_Details :

Please download the detailed product insert for complete instructions for the successful use of this ELISA; Use only as directed.

 

Background_Info : NT3 is understood to be important in promote the survival of visceral and proprioceptive sensory neurons. It is a secreted protein that belongs to the NGF-beta family and is found in brain and peripheral tissues.

 

Clone_Name
(AB only, monoclonals) :

 

Datasheet_Link : http://www.biosensis.com/pdf_datasheet.php?products_id=1310

 

Description (Long Description) : 

The Biosensis NT3 Rapid^TM enzyme-linked immune-sorbent assay (ELISA) Kit is a sandwich ELISA that allows the specific, fast and reliable quantification of NT3 in less than 4 hours in cell culture supernatants and human plasma (EDTA and citrate) only if used as directed. Please refer to the kit protocol for specific use instructions for each substrate application, in particular human plasma. Accurate quantification of NT3 in human plasma requires a heterophilic antibody (HA) blocker which can be purchased separately (BL-004-500).

This ELISA kit consists of a pre-coated polyclonal anti-NT3 capture antibody, a biotinylated monoclonal anti-NT3 detection antibody and horseradish peroxidase (HRP)-conjugated streptavidin. The addition of a substrate (3,3€™,5,5€™-tetramethylbenzidine, TMB) yields a colored reaction product, which is directly proportional to the concentration of NT3 present in samples and protein standards.

This NT3 Rapid^TM ELISA kit employs a recombinant human NT3 standard. NT3 is highly conserved and nearly identical in many species. The assay's capture and detection antibodies detect NT3 from other species including mouse, rat and monkey, thus it is expected that this NT3 Rapid^TM ELISA will also react with those species and possibly more because of the conversation of the immunogen used.

The antibodies used in this kit bind to epitopes within the mature domain of NT3. Thus, this ELISA kit may detect the full-length pro-form of NT3.

This kit has not been tested for other applications. Sufficient amount of NT3 standard is supplied to allow for spike- and recovery experiments in order to validate this ELISA assay for other sample matrices if required. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.

 

Expiry_Date : 

12 months from purchase.

 

Format (Form) : 

Ready to use Assay, complete with pre-coated plates, Concentrated Buffers, Detection reagents and complete instructions.

 

Immunogen (Antigen) :Human recombinant NT3, made in E.coli

 

Kit_Components : The ELISA kit box contains 2 x 96-well pre-coated strip plates, protein standards, detection reagents, wash and sample buffers, substrate buffer and detailed protocols.

 

MSDS (File name) :