Nestin Antibody

Host: Rabbit

Antibody Type: Polyclonal

Isotype: Rabbit IgG

Antibody Form: Affinity Purified Antibody

Immunogen Sequence: Synthetic peptide selected from the N-terminal region of human Nestin

Accession#: P48681

Gene ID: 10763

Appearance: Colorless liquid

Concentration: 0.25 mg/ml

Formulation: 100 µg (0.25 mg/ml) purified rabbit Ig polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.

Purification: Affinity purified

Species Reactivity: hu, ms

Application: ELISA/WB/IHC/IFC

Application & Usage: The antibody can be used for ELISA (0.25 µg/ml), Western Blotting (2.5 – 5.0 µg/ml), Immunohistochemistry (2.5 – 5.0 µg/ml) and Immunofluorescence (5 - 25 µg/ml).

Storage Temp.: -20 °C

Shipping : gel pack

Shelf Life: 12 months

Handling: The antibody solution should be gently mixed before use.

Brief Background Descriptions: Nestin is a class VI intermediate filament protein expressed predominantly in stem cells of the neural tube but absent from virtually all differentiated CNS cells. In the CNS, Nestin is downregulated upon differentiation and replaced by neurofilaments. Transient expression of nestin has been postulated as a key step committing cells to the neural differentiation pathway. Nestin expression has also been observed in pancreatic hematopoietic stem cell populations.