Proteins and Peptides
NES Antibody (LS-B2777)
- 貨號 Cat. No.
- LS-B2777
- 規格 Size
- 50 µl
- 品牌 Brand
- LSBio
Description
| Antibody Catalog ID: | LS-B2777 |
|---|---|
| Antibody Validation: | This antibody replaces catalog number LS-C73314. It has been validated for use in the following assays: IHC-P. |
| Antibody Target: | NES |
| Synonyms: | NES Antibody, FLJ21841 Antibody, Nbla00170 Antibody, nestin Antibody |
| Host: | NES antibody was produced in Mouse |
| Antigen Species: | NES antibody was raised against Human |
| Clonality: | Monoclonal |
| Isotype: | IgG1,k |
| Antigen Type: | Protein |
| Immunogen: | NES antibody was raised against a fragment of the human nestin protein corresponding to residues 1464-1614. |
| Antibody Specificity: | This antibody is specific for human nestin. Species cross-reactivity: This antibody recognizes human nestin protein. It does not cross-react with rodent protein. |
| Antigen Modification: | aa1464-1614 |
| Non-reactive Species: | Rodents |
| Purification: | Protein G Column |
| Recommended Storage: | Long term: Add glycerol (40-50%) -20°C; Short term: +4°C |
| Antibody Usage Summary: | Immunohistochemistry: LS-B2777 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody LS-D2, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2777 was determined to be 5 ug/ml. Western Blot: 1:1000-1:5000. Doublet band seen ~220-240kD. |
| Antibody Uses: | Immunohistochemistry - Paraffin (IHC-P) (5 µg/ml), Immunohistochemistry - Frozen (IHC-Fr), Immunocytochemistry (ICC), Western blot (WB) (1:1000 - 1:5000), Immunofluorescence (IF) (Optimal dilution to be determined by the researcher) |
| Size: | 50 µl |
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