NEK2 Antibody (LS-B129)

貨號 Cat. No.: LS-B129
規格 Size: 50 Âµg
品牌 Brand: LSBio

Description

Antibody Catalog ID:	LS-B129
Antibody Validation:	This antibody replaces catalog number LS-C18891. It has been validated for use in the following assays: IHC.
Antibody Target:	NIMA (Never In Mitosis Gene A)-Related Kinase 2 (NEK2)
Synonyms:	NEK2, HsPK 21, NEVER IN MITOSIS GENE A-RELATED KINASE 2, NIMA (Never in Mitosis Gene A)-related Kinase 2, NimA-like protein kinase 1, NIMA-RELATED KINASE 2, NLK1, serine/threonine protein kinase nek2
Family:	Protein Kinase
Subfamily:	NEK
Host:	NEK2 antibody was produced in Rabbit
Antigen Species:	NEK2 antibody was raised against Human
Clonality:	Polyclonal
Antigen Type:	Synthetic peptide - KLH conjugated
Immunogen:	NEK2 antibody was raised against synthetic peptide
Antibody Specificity:	aa 287-299 of Human NEK2 protein.
Antigen Modification:	aa287-299
Purification:	Immunoaffinity Chromatography
Presentation:	0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide.
Recommended Storage:	Store vial at -20 C prior to opening. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20 C or below. Avoid cycles of freezing and thawing.
Antibody Usage Summary:	Immunohistochemistry: LS-B129 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody LS-D1, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B129 was determined to be 2.5 ug/ml.
Antibody Uses:	Immunohistochemistry - Paraffin (IHC-P) (2.5 µg/ml), Western blot (WB) (1:500 - 1:2000), Immunofluorescence (IF), ELISA (1:2000 - 1:10000) (Optimal dilution to be determined by the researcher)
Size:	50 µg
Concentration:	1 mg/ml