NEDD8 AMC
- 貨號 Cat. No.
- UL-552
- 規格 Size
- 50 μg
- 品牌 Brand
- Boston Biochem
Description
NEDD8 AMC
human recombinant
Product Information
50 μg
X mg/ml (X μM) in 100 % DMSO Concentration varies with lot number.
9 kDa
> 95% by HPLC
Use & Storage
Substrate concentrations for assay range from 0.1-1 μM. Typical enzyme concentrations for UCH-L3 are 10-100 pM and for Isopeptidase-T are 10-100 nM. Release of AMC fluorescence by UCH enzymes can be monitored using Ex380 nm and Em460 nm wavelengths, respectively.
Store at -80°C. Avoid multiple freeze/thaw cycles.
Catalog #: UL-552
NEDD8 AMC
Product Source:
human recombinant
Fluorogenic substrate for NEDD8 hydrolases based on the carboxy-terminus derivatization of NEDD8 with 7-amido-4-methylcoumarin (AMC). NEDD8 AMC is useful for studying NEDD8 hydrolases when detection sensitivity or continuous monitoring of activity is essential.
Product Information
Quantity:
50 μg
Stock:
X mg/ml (X μM) in 100 % DMSO Concentration varies with lot number.
MW:
9 kDa
Purity:
> 95% by HPLC
Use & Storage
Use:
Substrate concentrations for assay range from 0.1-1 μM. Typical enzyme concentrations for UCH-L3 are 10-100 pM and for Isopeptidase-T are 10-100 nM. Release of AMC fluorescence by UCH enzymes can be monitored using Ex380 nm and Em460 nm wavelengths, respectively.
Storage:
Store at -80°C. Avoid multiple freeze/thaw cycles.