NEDD8 Agarose
- 貨號 Cat. No.
- UL-840
- 規格 Size
- 0.5 ml
- 品牌 Brand
- Boston Biochem
Description
NEDD8 Agarose
human recombinant
Product Information
0.5 ml
5 mg/ml resin (0.55 mM). Nedd8-agarose is supplied in Hepes buffered solution.
> 95% by SDS-PAGE
Use & Storage
Catalog #: UL-840
NEDD8 Agarose
Product Source:
human recombinant
NEDD8 is covalently coupled to agarose beads via primary amines allowing for a fully functional C-terminus. Useful for affinity binding of Nedd8 activating E1 enzyme, the Nedd8 carrier enzyme Ubch12, NEDD8 E3 ligases, and other proteins/enzymes that have an affinity for NEDD8. NEDD8 is poly-His6 tagged. The ubiquitin-like protein NEDD8 is conjugated to targets by the NEDD8-specific E1 activating enzyme (AppBp1/Uba3), the Ubch12 E2 enzyme, and the ROC1/Rbx1 RING finger E3 ligase. NEDD8 plays a critical regulatory role in cell proliferation and development, and modifies nearly all members of the cullin family.
Product Information
Quantity:
0.5 ml
Stock:
5 mg/ml resin (0.55 mM). Nedd8-agarose is supplied in Hepes buffered solution.
Purity:
> 95% by SDS-PAGE
Use & Storage
Use:
Equilibrate resin by washing with 5-10 ml desired start buffer. Binding and elution of material is dependent on individual experimental conditions.
Storage:
NEDD8-agarose can be re-used for at least 10-15 applications if properly maintained. After application, clean resin by washing with 10 ml 50 mM Tris-Cl pH 9.0, 1 M KCl. Remove cleaning solution by equilibrating resin in 25 mM HEPES (or similar buffer) pH 7.5, containing up to 1 mM NaN3. Resin should be stored at 4°C. DO NOT FREEZE