Proteins and Peptides
NEDD4 Antibody (LS-B2327)
- 貨號 Cat. No.
- LS-B2327
- 規格 Size
- 50 µl
- 品牌 Brand
- LSBio
Description
| Antibody Catalog ID: | LS-B2327 |
|---|---|
| Antibody Validation: | This antibody replaces catalog number LS-C37485. It has been validated for use in the following assays: IHC-P. |
| Antibody Target: | Nedd-4 Protein (NEDD4) |
| Synonyms: | NEDD4, nedd-4 protein |
| Host: | NEDD4 antibody was produced in Rabbit |
| Antigen Species: | NEDD4 antibody was raised against Rat |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Antigen Type: | Fusion protein |
| Immunogen: | NEDD4 antibody was raised against gST fusion protein corresponding to the WW2 domain (aa395-462) of rat Nedd4. |
| Antibody Specificity: | Recognizes rat Nedd4, Mr 115kD. Degradation products were also detected at Mr 55 and 60kD. Species cross-reactivity: mouse and human. |
| Antigen Modification: | aa395-462 |
| Predicted Species Reactivity: | Human, Mouse |
| Purification: | Immunoaffinity Chromatography |
| Presentation: | 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide and 5mg/ml BSA before the addition of glycerol to 30% |
| Recommended Storage: | +4°C or -20°C, Avoid repeated freezing and thawing. |
| Antibody Usage Summary: | Immunohistochemistry: LS-B2327 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody LS-D1, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2327 was determined to be 1:100-1:200. Positive control: Kidney cytoplasmic extracts. |
| Antibody Uses: | Immunohistochemistry - Paraffin (IHC-P) (1:100 - 1:200), Western blot (WB) (Optimal dilution to be determined by the researcher) |
| Size: | 50 µl |
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