Proteins and Peptides
NCF4 Antibody (LS-B2624)
- 貨號 Cat. No.
- LS-B2624
- 規格 Size
- 50 µg
- 品牌 Brand
- LSBio
Description
| Antibody Catalog ID: | LS-B2624 |
|---|---|
| Antibody Validation: | This antibody replaces catalog number LS-C9420. It has been validated for use in the following assays: IHC-P. |
| Antibody Target: | P40phox (NCF4) |
| Synonyms: | NCF4, MGC3810, NCF, NCF-4, P40-phox, P40PHOX, SH3PXD4 |
| Host: | NCF4 antibody was produced in Rabbit |
| Antigen Species: | NCF4 antibody was raised against Mouse |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Antigen Type: | Fusion protein |
| Immunogen: | NCF4 antibody was raised against his tagged fusion protein corresponding to the full length mouse p40-phox |
| Antibody Specificity: | Recognizes mouse p40-phox, Mr ~40kD. |
| Purification: | Protein A Column |
| Presentation: | 0.1 M Tris-glycine, pH 7.4, 0.15 M sodium chloride, 0.05% sodium azide, before the addition of glycerol to 30% |
| Recommended Storage: | +4°C or -20°C, Avoid repeated freezing and thawing. |
| Antibody Usage Summary: | Immunohistochemistry: LS-B2624 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody LS-D1, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2624 was determined to be 5 ug/ml. Western Blot: 0.5-2.0 ug/ml detects p40-phox in RIPA lysates from RAW 264.7 cells. |
| Antibody Uses: | Immunohistochemistry - Paraffin (IHC-P) (5 µg/ml), Western blot (WB) (0.5 - 2 µg/ml) (Optimal dilution to be determined by the researcher) |
| Size: | 50 µg |
| Concentration: | 1 mg/ml |
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