NCF1 / p47phox Antibody (LS-B2549)

貨號 Cat. No.: LS-B2549
規格 Size: 50 Âµg
品牌 Brand: LSBio

Description

Antibody Catalog ID:	LS-B2549
Antibody Validation:	This antibody replaces catalog number LS-C9426. It has been validated for use in the following assays: IHC-P.
Antibody Target:	NCF1 / p47phox
Synonyms:	NCF1 Antibody, FLJ79451 Antibody, NCF1A Antibody, neutrophil cytosol factor 1 Antibody, NOXO2 Antibody, P47phox Antibody, SH3PXD1A Antibody
Host:	NCF1 antibody was produced in Rabbit
Antigen Species:	NCF1 antibody was raised against Mouse
Clonality:	Polyclonal
Isotype:	IgG
Antigen Type:	Synthetic peptide
Immunogen:	NCF1 antibody was raised against kLH-conjugated, synthetic peptides containing the first 15 amino acids of the N-terminal and the last 15 amino acids of the C-terminus of mouse p47-phox.
Antibody Specificity:	Recognizes mouse p47-phox, Mr ~47kD.
Antigen Modification:	N-Terminus
Purification:	Protein A Column
Presentation:	0.1 M Tris-glycine, pH 7.4, 150 mM sodium chloride, 0.05% sodium azide.
Recommended Storage:	Long term: -20°C; Short term: +4°C
Antibody Usage Summary:	Immunohistochemistry: LS-B2549 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody LS-D1, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2549 was determined to be 10 ug/ml. Western Blot: 0.5-2 ug/ml detected p47-phox in RIPA lysates from RAW 264.7 cells.
Antibody Uses:	Immunohistochemistry - Paraffin (IHC-P) (10 µg/ml), Western blot (WB) (0.5 - 2 µg/ml) (Optimal dilution to be determined by the researcher)
Size:	50 µg
Concentration:	1 mg/ml