NAK antibody

Product Name 

NAK antibody

Product type 

Primary antibodies

Description 

Rabbit polyclonal to NAK

Immunogen 

Synthetic peptide (ERFGSLTMDGGLRNVD) corresponding to amino acids 712 to 727 of human NAK/TBK1, which are identical to those of mouse homologue.

BLAST 'ERFGSLTMDGGLRNVD'

Hu, Ms

Specificity 

No cross reactivity to IKKa,IKKb, IKKg, or IKKe.

ICC/IF, WB

Recommended dilutions

ICC/IF: Use at a concentration of 10 µg/ml.

WB: Use at a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 84 kDa.





Not yet tested in other applications.

Optimal dilutions/concentrations should be determined by the end user.

MOLT-4 whole cell lysate

Cellular localization 

Cytoplasmic

Relevance 

NFkappaB-activating kinase (NAK) also known as TANK binding kinase-1 (TBK1) or tumor necrosis factor receptor-associated factor 2-associated kinase (T2K) is a serine/threonine protein that belongs to the IkB kinase (IKK) family. Similar to IKK alpha and beta, NAK induces IkB degradation and NF-kB activity. NAK is activated by phorbol ester tumour promoters and growth factors, whereas catalytically inactive NAK specifically inhibits activation of NF-kB by protein kinase C-epsilon (PKCepsilon). NAK specifically phosphorylates IkB alpha on Serine 36 and NFkB subunit RelA (p65) on Serine 536. NAK is also known to interact with TANK, TRAF-2 and TRIF.

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

IgG fraction

PBS with 0.02% sodium azide

Material safety datasheet (MSDS) for this product:

Sodium Azide MSDS

Form 

Liquid

Concentration 

0.500 mg/ml

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

NAK antibody images:

References for NAK antibody (ab8121)

  This product has been used in:



McCoy CE et al. Glucocorticoids Inhibit IRF3 Phosphorylation in Response to Toll-like Receptor-3 and -4 by Targeting TBK1 Activation. J Biol Chem 283:14277-85 (2008). IP; Human. PubMed: 18356163