NADP/NADPH Quantitation Colorimetric Kit
Kits
- 貨號 Cat. No.
- K347-100
- 規格 Size
- K347-100
- Sample Type
- as well as many other biological fluids 、 Cell and tissue lysates 、 culture media 、 Plasma and Serum 、 urine
Description
Alternate Name :
Tag Line :
No NAD or NADH Detection
Storage Conditions: -20°C
Shelf life: 12 months
Summary :
• Detection method- Absorbance (450 nm)
• Species reactivity- Mammalian
• Applications- Convenient tool for sensitive detection of the intracellular nucleotides: NADP, NADPH and their ratio
Detection Method:
Features & Benefits:
• Simple procedure; takes ~2 hours
• Fast and convenient
• Kit contains the necessary reagents for accurate measurement of NADP and NADPH and their ratio
• Enzymes in the system specifically recognize NADP/NADPH in an enzyme cycling reaction (It does not recognize NADᶧ/NADH)
Description:
Assays of nicotinamide nucleotides are of continual interest in the studies of energy transforming and redox state of cells or tissue. The NADP/NADPH Quantification Kit provides a convenient tool for sensitive detection of the intracellular nucleotides: NADP, NADPH and their ratio. The enzymes in the system specifically recognize NADP/NADPH in an enzyme cycling reaction (It does not recognize NADᶧ/NADH). There is no need to purify NADP/NADPH from sample mix. The enzyme cycling reaction significantly increases detection sensitivity. Results can be quantified using plate reader at OD450 nm.