Proteins and Peptides
MYH9 Antibody (LS-B2802)
- 貨號 Cat. No.
- LS-B2802
- 規格 Size
- 50 µl
- 品牌 Brand
- LSBio
Description
| Antibody Catalog ID: | LS-B2802 |
|---|---|
| Antibody Validation: | This antibody replaces catalog number LS-C73063. It has been validated for use in the following assays: IHC-P. |
| Antibody Target: | MYH9 |
| Synonyms: | MYH9 Antibody, DFNA17 Antibody, EPSTS Antibody, FTNS Antibody, human non-muscle myosin heavy chain [fragment] Antibody, human nonmuscle myosin heavy chain nmhc Antibody, MGC104539 Antibody, MHA Antibody, NMHC-II-A Antibody, NMMHCA Antibody |
| Host: | MYH9 antibody was produced in Rabbit |
| Antigen Species: | MYH9 antibody was raised against Human |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Antigen Type: | Synthetic peptide |
| Immunogen: | MYH9 antibody was raised against synthetic peptide conjugated to KLH, corresponding to amino acids 1950-1961 of human non-muscle Myosin IIA. |
| Antibody Specificity: | Recognizes human myosin IIA. Species cross-reactivity: Cross-reacts rat and canine. |
| Antigen Modification: | aa1950-1961 |
| Predicted Species Reactivity: | Canine, Rat |
| Purification: | Immunoaffinity Chromatography |
| Presentation: | PBS, pH 7.4, 1% BSA, 15 mM sodium azide. |
| Recommended Storage: | +4°C or -20°C, Avoid repeated freezing and thawing. |
| Antibody Usage Summary: | Immunohistochemistry: LS-B2802 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody LS-D1, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2802 was determined to be 2.5 ug/ml. Western Blot: 1:1000. Detects a band at ~200kD. Staining of myosin IIA (nonmuscle) by immunoblotting is inhibited by the immunizing peptide. Additional bands may be detected in some preparations. Positive control: MDCK or Jurkat whole cell extract (Western Blot). NRK cells (Immunofluorescence). |
| Antibody Uses: | Immunohistochemistry - Paraffin (IHC-P) (2.5 µg/ml), Western blot (WB) (1:1000), Immunoprecipitation (IP), Immunofluorescence (IF) (1:500) (Optimal dilution to be determined by the researcher) |
| Size: | 50 µl |
| Concentration: | 1 mg/ml |
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