Myc-Ubiquitin
- 貨號 Cat. No.
- U-115
- 規格 Size
- 1 mg
- 品牌 Brand
- Boston Biochem
Description
Myc-Ubiquitin
human recombinant
Product Information
1 mg
9.3 kDa
> 95% by SDS-PAGE
Aqueous solutions up to 10 mg/ml
This is a lyophilized powder
Use & Storage
Lyophilized powder at 4 degrees C. Solubilized stock solution at -20 degrees C. Avoid multiple freeze/thaw cycles.
Catalog #: U-115
Myc-Ubiquitin
Product Source:
human recombinant
This fully functional N-terminal myc-tagged ubiquitin protein allows for the convenient detection or affinity purification of ubiquitinated proteins in vitro. The tag sequence (EQKLISEEDL) is an epitope derived from the C-terminal region of the human proto-oncoprotein p62c-myc. This tag is specifically recognized by anti-myc antibodies or anti-myc-agarose. Ubiquitin is a 76 amino acid, highly conserved nuclear and cytoplasmic protein. It is found exclusively in eukaryotes, becomes covalently attached to substrate proteins by enzymes in the Ubiquitin-Proteasome Pathway (UPP) and has a major role in targeting cellular proteins for the ATP-dependent degradation by the 26S proteasome. Ubiquitination also affects proteasomeindependent events such as protein localization, activity and function.
Product Information
Quantity:
1 mg
MW:
9.3 kDa
Purity:
> 95% by SDS-PAGE
Solubility:
Aqueous solutions up to 10 mg/ml
Supplied:
This is a lyophilized powder
Use & Storage
Use:
Typical concentration to support in vitro conjugation is 500 μM to 1 mM depending on conditions
Storage:
Lyophilized powder at 4 degrees C. Solubilized stock solution at -20 degrees C. Avoid multiple freeze/thaw cycles.