MYC / c-Myc Antibody (LS-B2791)

貨號 Cat. No.: LS-B2791
規格 Size: 50 Âµg
品牌 Brand: LSBio

Description

Antibody Catalog ID:	LS-B2791
Antibody Validation:	This antibody replaces catalog number LS-C46098. It has been validated for use in the following assays: IHC-P.
Antibody Target:	MYC / c-Myc
Synonyms:	MYC, BHLHe39, c-myc, MRTL, myc, myc proto-oncogene, rats1, v-myc
Host:	MYC antibody was produced in Mouse
Antigen Species:	MYC antibody was raised against Human
Clonality:	Monoclonal
Isotype:	IgG1
Antibody Clone Name:	9E10
Antigen Type:	Synthetic peptide
Immunogen:	MYC antibody was raised against synthetic peptide sequence (AEEQKLISEEDLL) corresponding to the C-terminal region of human c-myc
Antibody Specificity:	The antibody 9E10 may be used to detect the c-Myc tag. The c-myc gene (8q24 on human chromosome) is the cellular homologue of the v-myc gene originally isolated from an avian myelocytomatosis virus. The c-Myc protein is a transcription factor (nuclear localization). c-Myc is commonly activated in a variety of tumor cells and plays an important role in cellular proliferation, differentiation, apoptosis and cell cycle progression. The phosphorylation of c-Myc has been investigated and previous studies have suggested a functional association between phosphorylation at Thr58/Ser62 by glycogen synthase kinase 3, cyclin-dependent kinase, ERK2 and C-Jun N-terminal Kinase (JNK) in cell proliferation and cell cycle regulation. In normal cells the expression of c-Myc is tightly regulated but in human cancers c-Myc is frequently deregulated. c-Myc is also essential for tumor cell development in vasculogenesis and angiogenesis that distribute blood throughout the cells
Antigen Modification:	C-Terminus
Purification:	Protein G Column
Presentation:	PBS, 15 mM sodium azide, approx., pH 7.4.
Recommended Storage:	+4°C or -20°C, Avoid repeated freezing and thawing.
Antibody Usage Summary:	Immunohistochemistry: LS-B2791 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody LS-D2, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2791 was determined to be 10 ug/ml.
Antibody Uses:	Immunohistochemistry - Paraffin (IHC-P) (10 µg/ml), Western blot (WB) (0.5 - 2 µg/ml), Immunoprecipitation (IP) (1 - 5 µg/ml), Flow Cytometry (Flo) (1 - 5 µg/ml) (Optimal dilution to be determined by the researcher)
Size:	50 µg
Concentration:	1 mg/ml