Murine Leptin

SOURCE: E. coli

PURITY: >98% by SDS-PAGE Endotoxin level is <0.1 ng per ug of Leptin.

MOL. WEIGHT: 16.2 kDa

FORM: Lyophilized with no additive

RECONSTITUTION: Centrifuge the vial prior to opening. Reconstitute in 10 mM HCl to a concentration of 1 mg/ml. The solution can then be diluted into other aqueous buffers and stored at 4oC for 1 week or -20oC for future use.

STORAGE CONDITIONS: The lyophilized protein is best stored at -20oC. Reconstituted Leptin should be stored at working aliquots at -20oC.

Leptin (Anti-Obesity Protein) is the protein product of the ob (obese) gene in mice and seems to be involved in appetite control. The ob gene is expressed in adipose tissue and is thought to regulate the body's fat stores. Mice with the ob/ob genotype also develop a form of diabetes similar to type II (non-insulin dependent) diabetes. Murine Leptin is a 16 kDa protein containing 147 amino acid residues.

DESCRIPTION:

BIOLOGICAL ACTIVITY: Determined in an in vivo assay using the ob/ob mouse obesity model and NZO. Both strains of mice were treated via intraperitoneal injection once daily at a dose of 5 mg leptin/gm of body weight for 7 days. Significant effects on body weight, food consumption, and plasma glucose levels were observed to saline-treated controls.

USAGE: For Research Purpose Only. Not to be used in humans.