Murine FGF-9

SOURCE: E. coli

PURITY: >98% by SDS-PAGE and HPLC analyses Endotoxin level is <0.1 ng per ug of FGF-9.

MOL. WEIGHT: 23.3 kDa

FORM: Sterile filtered and lyophilized in 10 mM Tris, pH 8.0 + 0.15 M Ammonium Sulfate.

RECONSTITUTION: Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.2-0.5 mg/ml. This solution can then be diluted into other aqueous buffers or stored at 4oC for 1 week or -20oC for future use. Repeated freeze/thaw cycles will result in some loss of activity.

STORAGE CONDITIONS: The lyophilized FGF-9 should be is stored desiccated below 0oC. Reconstituted FGF-9 should be stored at working aliquots at -20oC.

FGF-9 is a heparin binding growth factor that belongs to the FGF family. FGF-9 stimulates the proliferation and activation of glial cells and other cells that express FGF receptors. Recombinant murine FGF-9 is a 23.3 kDa protein containing 205 amino acid residues.

DESCRIPTION:

BIOLOGICAL ACTIVITY: The ED50 determined by the dose-dependent stimulation of thymidine uptake by BaF3 cells expressing FGF receptors is <0.5 ng/ml, corresponding to a Specific Activity of >2 x 10⁶ units/mg.

NOTE:

FOR RESEARCH USE ONLY! NOT TO BE USED IN HUMANS.