Murine Adiponectin

SOURCE: E. coli

PURITY: >90% by SDS-PAGE Endotoxin level is <0.1 ng per ug.

FORM: Lyophilized from a 0.2 um filtered solution in 25 mM Tris, 0.15 M NaCl, pH 7.4 containing 50 ug of bovine serum albumin (BSA) per 1 ug of adiponectin.

RECONSTITUTION: We recommend a quick spin followed by reconstitution in PBS containing at least 0.1% BSA to a concentration of 0.1-1.0 mg/ml. This stock solution can then be diluted into other aqueous buffers and stored at 4oC for 1 week or -20oC for future use.

STORAGE CONDITIONS: The lyophilized protein is best-stored desiccated below 0oC. Reconstituted Adiponectin should be stored in working aliquots at -20oC or -70oC.

Adiponectin (also called Acrp30, AdipoQ) is an adipocyte specific secreted protein that circulates in the plasma. It is induced during adipocyte differentiation and it secretion is stimulated by insulin. Mouse adiponectin shares about 83% amino acid identity with that of human. Adiponectin plays a role in various physiological processes such as energy homeostasis and obesity. Plasma levels of adiponectin are reduced in obese humans, and decreased levels are associated with insulin resistance and hyperinsulinemia. ACTIVITY: Measured by its ability to inhibit proliferation of mouse myeloid cell line M1 (Yokota, T. et al., Blood 96:1723, 2000). The ED50 for the effect is typically 1-7 ug/ml. For Research Use Only! Not for Use in Humans.

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