MuRF-1 Auto-Ubiquitination Kit
- 貨號 Cat. No.
- K-100
- 規格 Size
- 1 kit
- 品牌 Brand
- Boston Biochem
Description
MuRF-1 Auto-Ubiquitination Kit
human recombinant
Product Information
1 kit
Use & Storage
Store at -80°C. Avoid multiple freeze/thaw cycles.
Catalog #: K-100
MuRF-1 Auto-Ubiquitination Kit
Product Source:
human recombinant
This kit is designed to perform E3 ligase auto-ubiquitination reactions in vitro, which requires the activities of the ubiquitin E1 activating enzyme (E-305), the E2 conjugating enzyme UbcH5c (E2-627) and E3 ligase MurF-1 (E3-100). The E1 enzyme charges the ubiquitin by forming an ATP-dependent high energy thiolester bond. The activated ubiquitin is subsequently transferred to UbcH5c then to the MurF-1. The MurF-1-S-Ub complex has the ability to both auto-ubiquitinate itself and/or transfer the Ub to protein substrates (eg. troponin). Alternatively labeled Ub proteins may be substituted for biotin-ubiquitin for detection such as fluorescein-ubiquitin (U-590) and rhodamine-ubiquitin (U-600).
NOTE: Kit contains reagents sufficient for 10 x 20 μl reactions. Concentration of components vary with Lot #.
Product Information
Quantity:
1 kit
Supplied:
| Concentration | Volume | |
| 10X E1 Enzyme | X mg/ml (X μM) | 20 μl |
| 10X UbcH5c | X mg/ml (X μM) | 20 μl |
| 5X His6-MuRF-1 | X mg/ml (X μM) | 40 μl |
| 10X Biotin-Ubiquitin | X mg/ml (X μM) | 20 μl |
| 10X Reaction Buffer | X mM | 20 μl |
Background:
MurF1 (Muscle-specific RING-Finger protein 1) is a RING-finger E3 ligase found in striated muscle (heart and skeletal) and iris tissues. MurF1 shares 62% and 77% sequence homology with MurF2 and MurF3 respectively. The protein contains a RING-finger/B-box/coiled-coil tripartite fold known as TRIM, and it can form homo- and hetero-oligomers vial the coiled-coil dimerization motifs. The N-terminal E3 ligase activity regulates the proteasomal degradation of cardiac troponin and probably other sarcomeric-associated proteins. The exact cellular roles and substrates for MurF1 are not yet known but it interacts with various proteins including titin, isopeptidase3, SUMO-3, UbcH9 and GMEB-1. MurF1 may have a role in muscle adaptation, atrophy and hypertrophy mediated by ubiquitin-proteasome pathways (UPP). Muscle atrophy is associated with many diseases including cancer, diabetes and AIDS.
Use & Storage
Storage:
Store at -80°C. Avoid multiple freeze/thaw cycles.