Proteins and Peptides
MUC1 Antibody (LS-B2244)
- 貨號 Cat. No.
- LS-B2244
- 規格 Size
- 50 µg
- 品牌 Brand
- LSBio
Description
| Antibody Catalog ID: | LS-B2244 |
|---|---|
| Antibody Validation: | This antibody replaces catalog number LS-C16634. It has been validated for use in the following assays: IHC-P. |
| Antibody Target: | Mucin 1 (MUC1/CA15-3/CA27-29) |
| Synonyms: | MUC1, Epithelial membrane antigen (EMA), mucin 1 |
| Family: | Mucin |
| Subfamily: | not assigned-Mucin |
| Host: | MUC1 antibody was produced in Mouse |
| Antigen Species: | MUC1 antibody was raised against Human |
| Clonality: | Monoclonal |
| Isotype: | IgG3 |
| Modifications: | Fluorescein (FITC) |
| Immunogen: | MUC1 antibody was raised against urinary MUC-1 mucin. |
| Antibody Specificity: | Recognizes human CD227, the breast cancer associated mucin encoded by the Muc-1 gene. In normal tissues, expression is restricted to specialized glandular epithelial cells. Recognizes the peptide epitope Arg-Pro-Ala-Pro within the protein core of the mucin. |
| Purification: | Protein G Column |
| Presentation: | PBS, pH 7.4, 0.09% sodium azide, 1% BSA |
| Recommended Storage: | Long term: -20°C; Short term: +4°C |
| Antibody Usage Summary: | Immunohistochemistry: LS-B2244 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody LS-D2, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2244 was determined to be 1:50. Flow Cytometry: Neat-1:10. Use 10 ul to label 10^6 cells in 100ul. |
| Antibody Uses: | Immunohistochemistry - Paraffin (IHC-P) (1:50), Flow Cytometry (Flo) (Optimal dilution to be determined by the researcher) |
| Size: | 50 µg |
| Concentration: | 0.1 mg/ml |
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