MTT Cell Proliferation Assay Kit (Colorimetric)

Alternate Name :

Tag Line :

Non-radioactive and high-throughput method to measure cell proliferation/viability/cytotoxicity

Storage Conditions: -20°C

Shelf life: 12 months

Summary :

• Detection- Absorbance (490 nm)
• Applications: - Measurement of cell proliferation in response to growth factors, cytokines, mitogens and nutrients
- Analysis of cytotoxic and cytostatic compounds such as anticancer drugs, toxic agents and other pharmaceuticals
- Assessment of physiological mediators that inhibit cell growth

Detection Method: Absorbance (490 nm)

Features & Benefits:

• Non-radioactive
• High-throughput
• The entire assay can be performed directly in a 96-well plate and does not require washing/harvesting/or solubilization steps.

Description:

Quantification of the number of viable cells is an indispensable tool in cell biology research. BioVision’s MTT Cell Proliferation Assay Kit is a sensitive method for quantification of viable cells in proliferation and cytotoxicity assay. The method is based on the conversion of water soluble MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) compound to an insoluble formazan product. Viable cells with active metabolism convert MTT into formazan, however, dead cells lose this ability. Thus color formation serves as a useful and convenient marker of only the viable cells. The measured absorbance (590 nm) is proportional to the number of viable cells. This assay kit provides an easy-to-use, non-radioactive, and high-throughput method for cell proliferation, cell viability, chemotaxis, cytotoxicity, and apoptosis.