Proteins and Peptides
MSLN Antibody (LS-B2618)
- 貨號 Cat. No.
- LS-B2618
- 規格 Size
- 50 µg
- 品牌 Brand
- LSBio
Description
| Antibody Catalog ID: | LS-B2618 |
|---|---|
| Antibody Validation: | This antibody replaces catalog number LS-C25601. It has been validated for use in the following assays: IHC-P. |
| Antibody Target: | MSLN |
| Synonyms: | MSLN Antibody, cak1 antigen Antibody, mesothelin Antibody, MPF Antibody, msln Antibody, SMRP Antibody |
| Host: | MSLN antibody was produced in Mouse |
| Antigen Species: | MSLN antibody was raised against Human |
| Clonality: | Monoclonal |
| Isotype: | IgG1 |
| Antigen Type: | Purified protein |
| Immunogen: | MSLN antibody was raised against human mesothelin. |
| Antibody Specificity: | Recognizes human mesothelin protein. Reactivity has been confirmed with SK-OV-3 cell lysates by Western blot, with living human OVCAR-32 cells and living cultured mesothelioma cells by immunofluorescence, and with frozen human mesothelia of peritoneal, pleural and pericardial cavities, as well as frozen ovarian non-mucinous tumors, squamous tumors of the esophagus, cervical cancer and frozen epithelial mesothelioma tissue by immunohistochemistry. |
| Purification: | Protein A Column |
| Presentation: | PBS, pH 7.4, 0.1% sodium azide. |
| Recommended Storage: | +4°C or -20°C, Avoid repeated freezing and thawing. |
| Antibody Usage Summary: | Immunohistochemistry: LS-B2618 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody LS-D2, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2618 was determined to be 10 ug/ml. |
| Antibody Uses: | Immunohistochemistry - Paraffin (IHC-P) (10 µg/ml), Immunohistochemistry - Frozen (IHC-Fr) (5 - 10 µg/ml), Western blot (WB) (1 - 3 µg/ml), Immunofluorescence (IF) (5 - 10 µg/ml) (Optimal dilution to be determined by the researcher) |
| Size: | 50 µg |
| Concentration: | 0.5 mg/ml |
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