Mouse Vascular endothelial growth factor A/VEGF-A/VEGF-164/VEGF-1/VEGF-120/VEGF-2 ELISA Kit (1 plate)

ZZZZ_Status (Active, Disc) : Active

 

AA_Supplier Name : Biosensis

 

Alternative_Name (Other Names) :

VEGF-A; Vascular permeability factor; VPF; VEGFA; VEGF; VEGF-1(VEGF164); VEGF-2(VEGF120)

 

Antibody_Isotype
(Antibody only) :

 

Application_Details :

Please download the detailed product insert for complete instructions for the successful use of this ELISA; Use only as directed.

 

Background_Info :

 

Clone_Name
(AB only, monoclonals) :

 

Datasheet_Link : http://www.biosensis.com/pdf_datasheet.php?products_id=1418

 

Description (Long Description) : 

The mouse Vascular endothelial growth factor (VEGF) Kit is a sandwich ELISA. The capture antibody is a VEGF antibody pre-coated onto the 96-well strip plates provided in the kit. Mouse test samples and standards of known VEGF concentration are added to these wells and allowed to complex with the bound VEGF antibody. A biotinylated anti-mouse VEGF antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of VEGF present in the samples. The purpose of this kit is the in-vitro quantitative determination of mouse VEGF in samples such as sera, plasma, tissue lysates and cell culture supernates. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.

Vascular endothelial growth factor (VEGF) is a disulfide linked homodimeric protein associated with angiogenesis, vasculogenesis and endothelial cell growth. At least 10 isoforms of VEGF are produced by alternative splicing with varying levels of expression. The isoforms VEGF188, VEGF164 and VEGF120 are widely expressed whereas the isoforms VEGF205 and VEGF144 are uncommon. Isoforms VEGF164 and VEGF120 are freely secreted whereas isoform VEGF188 remains cell associated.

 

Expiry_Date : 

This kit may be stored for up to 6 months at 2°C to 8°C from the date of shipment. Standards should be stored at -20°C or -80°C (recommended at –80°C) after reconstitution. Coated strips or reagents may be store for up to 1 month at 2°C to 8°C.

 

Format (Form) : 

Ready to use Assay, complete with pre-coated plates, Concentrated Buffers, Detection reagents and complete instructions.

 

Immunogen (Antigen) :The standard and immunogen used in the ELISA is recombinant mouse VEGF-A amino acids 27-190 produced in and purified from a SF21 insect cell expression system.

 

Kit_Components : The ELISA kit box contains 1 x 96-well pre-coated strip plate, protein standards, detection reagents, substrate buffer and detailed protocols.

 

MSDS (File name) :