Mouse Thrombopoietin/TPO/Megakaryocyte growth and development factor/MGDF ELISA Kit (2 plates)

ZZZZ_Status (Active, Disc) : Active

 

AA_Supplier Name : Biosensis

 

Alternative_Name (Other Names) :

TPO; C-mpl ligand; mL; Megakaryocyte colony-stimulating factor; Megakaryocyte growth and development factor; MGDF; Myeloproliferative leukemia virus oncogene ligand; Thpo;

 

Antibody_Isotype
(Antibody only) :

 

Application_Details :

Please download the detailed product insert for complete instructions for the successful use of this ELISA; Use only as directed.

 

Background_Info :

 

Clone_Name
(AB only, monoclonals) :

 

Datasheet_Link : http://www.biosensis.com/pdf_datasheet.php?products_id=844

 

Description (Long Description) : 

The Biosensis Mouse Thrombopoietin/TPO ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody specific for Thrombopoietin/TPO has been precoated onto 96-well plates. Standards and test samples are added to the wells, and then a biotinylated detection polyclonal antibody specific for Thrombopoietin/TPO is added subsequently followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates washed away again with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP driven enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that is changed into yellow by the addition of an acid stop solution. The intensity of this coloured product is directly proportional to the concentration of TPO present in the samples. The purpose of this kit is the in-vitro quantitative determination of mouse TPO in samples such as serum, EDTA or heparin treated plasma, tissue lysates and cell culture supernatants. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.

 

Expiry_Date : 

This kit may be stored for up to 6 months at 2°C to 8°C from the date of shipment. Standards should be stored at -20°C or -80°C (recommended at –80°C) after reconstitution. Coated strips or reagents may be store for up to 1 month at 2°C to 8°C.

 

Format (Form) : 

Ready to use Assay, complete with pre-coated plates, Concentrated Buffers, Detection reagents and complete instructions.

 

Immunogen (Antigen) :The standard and antigen for this mouse TPO ELISA is recombinant mouse TPO produced in a mammalian cell expression system and purified, aa.22-356 of mouse TPO.

 

Kit_Components : The ELISA kit box contains 2 x 96-well pre-coated strip plates, protein standards, detection reagents, substrate buffer and detailed protocols.

 

MSDS (File name) :