Mouse TFPI / LACI / EPI Protein (His Tag)

Species : Mouse

Synonyms : A630013F22Rik,AW552122,EPI,LACI

Host : HEK293 Cells

Purity :> 97 % as determined by SDS-PAGE

Activity : Measured by its ability to inhibit trypsin cleavage of a fluorogenic peptide substrate, Mca-RPKPVE-Nval-WRK(Dnp)-NH2 (Anaspec, Catalog # 27114).
The IC50 value is < 0.5 nM, as measured in 100£gL reaction mixture containing 1.25 ng trypsin (Sigma, Catalog # T4799), 10 £gM substrate, 50 mM Tris, 10 mM CaCl2 , 0.15 M NaCl, 0.05% Brij 35, pH 7.5
.